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EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway
المؤلفون المشاركون
Xie, Hua
Sun, Jianqin
Chen, Yanqiu
Zong, Min
Li, Shijie
Wang, Yan
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-11
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Background.
The aim of this study is to investigate whether (-)-epigallocatechin-3-gallate (EGCG) can prevent the UA-induced inflammatory effect of human umbilical vein endothelial cells (HUVEC) and the involved mechanisms in vitro.
Methods.
HUVEC were subjected to uric acid (UA) with or without EGCG treatment.
RT-PCR and western blots were performed to determine the level of inflammation marker.
The antioxidant activity was evaluated by measuring scavenged reactive oxygen species (ROS).
Functional studies of the role of Notch-1 in HUVEC lines were performed using RNA interference analyses.
Results.
UA significantly increased the expressions of IL-6, ICAM-1, TNF-α, and MCP-1 and the production of ROS in HUVEC.
Meanwhile, the expression of Notch-1 and its downstream effects significantly increased.
Using siRNA, inhibition of Notch-1 signaling significantly impeded the expressions of inflammatory cytokines under UA treatment.
Interestingly, EGCG suppressed the expressions of inflammatory cytokines and the generation of ROS.
Western blot analysis of Notch-1 showed that EGCG significantly decreased the expressions of inflammatory cytokines through Notch-1 signaling pathways.
Conclusions.
In summary, our findings indicated that Notch-1 plays an important role in the UA-induced inflammatory response, and the downregulation of Notch-1 by EGCG could be an effective approach to decrease inflammation and oxidative stress induced by UA.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xie, Hua& Sun, Jianqin& Chen, Yanqiu& Zong, Min& Li, Shijie& Wang, Yan. 2015. EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075562
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xie, Hua…[et al.]. EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075562
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xie, Hua& Sun, Jianqin& Chen, Yanqiu& Zong, Min& Li, Shijie& Wang, Yan. EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075562
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1075562
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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