EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway
Joint Authors
Xie, Hua
Sun, Jianqin
Chen, Yanqiu
Zong, Min
Li, Shijie
Wang, Yan
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-11
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Background.
The aim of this study is to investigate whether (-)-epigallocatechin-3-gallate (EGCG) can prevent the UA-induced inflammatory effect of human umbilical vein endothelial cells (HUVEC) and the involved mechanisms in vitro.
Methods.
HUVEC were subjected to uric acid (UA) with or without EGCG treatment.
RT-PCR and western blots were performed to determine the level of inflammation marker.
The antioxidant activity was evaluated by measuring scavenged reactive oxygen species (ROS).
Functional studies of the role of Notch-1 in HUVEC lines were performed using RNA interference analyses.
Results.
UA significantly increased the expressions of IL-6, ICAM-1, TNF-α, and MCP-1 and the production of ROS in HUVEC.
Meanwhile, the expression of Notch-1 and its downstream effects significantly increased.
Using siRNA, inhibition of Notch-1 signaling significantly impeded the expressions of inflammatory cytokines under UA treatment.
Interestingly, EGCG suppressed the expressions of inflammatory cytokines and the generation of ROS.
Western blot analysis of Notch-1 showed that EGCG significantly decreased the expressions of inflammatory cytokines through Notch-1 signaling pathways.
Conclusions.
In summary, our findings indicated that Notch-1 plays an important role in the UA-induced inflammatory response, and the downregulation of Notch-1 by EGCG could be an effective approach to decrease inflammation and oxidative stress induced by UA.
American Psychological Association (APA)
Xie, Hua& Sun, Jianqin& Chen, Yanqiu& Zong, Min& Li, Shijie& Wang, Yan. 2015. EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075562
Modern Language Association (MLA)
Xie, Hua…[et al.]. EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-10.
https://search.emarefa.net/detail/BIM-1075562
American Medical Association (AMA)
Xie, Hua& Sun, Jianqin& Chen, Yanqiu& Zong, Min& Li, Shijie& Wang, Yan. EGCG Attenuates Uric Acid-Induced Inflammatory and Oxidative Stress Responses by Medicating the NOTCH Pathway. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-10.
https://search.emarefa.net/detail/BIM-1075562
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075562