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Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor
المؤلفون المشاركون
Wang, Lingyan
Duan, Qi
Wang, Tingting
Ahmed, Mohamed
Zhang, Na
Li, Yongmei
Li, Lanying
Yao, Xiaomei
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-29
دولة النشر
مصر
عدد الصفحات
14
التخصصات الرئيسية
الملخص EN
A major source of reactive oxygen species (ROS) generation is the mitochondria.
By using flow cytometry of the mitochondrial fluorescent probe, MitoSOX Red, western blot of mitochondrial ROS scavenger Peroxiredoxin (Prx) 3 and fluorescence immunostaining, ELISA of cleaved caspases 3 and 9, and TUNEL staining, we demonstrated that exposure to 100 μM KI for 2 hours significantly increased mitochondrial superoxide production and Prx 3 protein expression with increased expressions of cleaved caspases 3 and 9.
Besides, we indicated that superoxide dismutase (SOD) at 1000 unit/mL attenuated the increase in mitochondrial superoxide production, Prx 3 protein expression, and lactate dehydrogenase (LDH) release and improved the relative cell viability at 100 μM KI exposure.
However, SOD inhibitor diethyldithiocarbamic acid (DETC) (2 mM), Rotenone (0.5 μM), a mitochondrial complex I inhibitor, and Antimycin A (10 μM), a complex III inhibitor, caused an increase in mitochondrial superoxide production, Prx 3 protein expression, and LDH release and decreased the relative cell viability.
We conclude that the inhibitors of mitochondrial respiratory chain complex I or III may be involved in oxidative stress caused by elevated concentrations of iodide, and SOD demonstrates its protective effect on the Fischer rat thyroid cell line (FRTL) cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wang, Lingyan& Duan, Qi& Wang, Tingting& Ahmed, Mohamed& Zhang, Na& Li, Yongmei…[et al.]. 2015. Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1075565
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wang, Lingyan…[et al.]. Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1075565
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wang, Lingyan& Duan, Qi& Wang, Tingting& Ahmed, Mohamed& Zhang, Na& Li, Yongmei…[et al.]. Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1075565
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1075565
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
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