Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor
Joint Authors
Wang, Lingyan
Duan, Qi
Wang, Tingting
Ahmed, Mohamed
Zhang, Na
Li, Yongmei
Li, Lanying
Yao, Xiaomei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-29
Country of Publication
Egypt
No. of Pages
14
Main Subjects
Abstract EN
A major source of reactive oxygen species (ROS) generation is the mitochondria.
By using flow cytometry of the mitochondrial fluorescent probe, MitoSOX Red, western blot of mitochondrial ROS scavenger Peroxiredoxin (Prx) 3 and fluorescence immunostaining, ELISA of cleaved caspases 3 and 9, and TUNEL staining, we demonstrated that exposure to 100 μM KI for 2 hours significantly increased mitochondrial superoxide production and Prx 3 protein expression with increased expressions of cleaved caspases 3 and 9.
Besides, we indicated that superoxide dismutase (SOD) at 1000 unit/mL attenuated the increase in mitochondrial superoxide production, Prx 3 protein expression, and lactate dehydrogenase (LDH) release and improved the relative cell viability at 100 μM KI exposure.
However, SOD inhibitor diethyldithiocarbamic acid (DETC) (2 mM), Rotenone (0.5 μM), a mitochondrial complex I inhibitor, and Antimycin A (10 μM), a complex III inhibitor, caused an increase in mitochondrial superoxide production, Prx 3 protein expression, and LDH release and decreased the relative cell viability.
We conclude that the inhibitors of mitochondrial respiratory chain complex I or III may be involved in oxidative stress caused by elevated concentrations of iodide, and SOD demonstrates its protective effect on the Fischer rat thyroid cell line (FRTL) cells.
American Psychological Association (APA)
Wang, Lingyan& Duan, Qi& Wang, Tingting& Ahmed, Mohamed& Zhang, Na& Li, Yongmei…[et al.]. 2015. Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1075565
Modern Language Association (MLA)
Wang, Lingyan…[et al.]. Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1075565
American Medical Association (AMA)
Wang, Lingyan& Duan, Qi& Wang, Tingting& Ahmed, Mohamed& Zhang, Na& Li, Yongmei…[et al.]. Mitochondrial Respiratory Chain Inhibitors Involved in ROS Production Induced by Acute High Concentrations of Iodide and the Effects of SOD as a Protective Factor. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1075565
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075565