Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro
المؤلفون المشاركون
Zhao, Hailin
Cui, Jian
Wang, Chunyan
Sun, James J.
Lu, Kaizhi
Ma, Daqing
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-03-08
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Background.
Oxidative stress plays a pivotal role in the lung injuries of critical ill patients.
This study investigates the protection conferred by α2 adrenoceptor agonist dexmedetomidine (Dex) from lung alveolar epithelial cell injury induced by hydrogen peroxide (H2O2) and the underlying mechanisms.
Methods.
The lung alveolar epithelial cell line, A549, was cultured and then treated with 500 μM H2O2 with or without Dex (1 nM) or Dex in combination with atipamezole (10 nM), an antagonist of α2 receptors.
Their effect on mitochondrial membrane potential (Δψm), reactive oxygen species (ROS), and the cell cycle was assessed by flow cytometry.
Cleaved-caspases 3 and 9, BAX, Bcl-2, phospho-mTOR (p-mTOR), ERK1/2, and E-cadherin expression were also determined with immunocytochemistry.
Results.
Upregulation of cleaved-caspases 3 and 9 and BAX and downregulation of Bcl-2, p-mTOR, and E-cadherin were found following H2O2 treatment, and all of these were reversed by Dex.
Dex also prevented the ROS generation, cytochrome C release, and cell cycle arrest induced by H2O2.
The effects of Dex were partially reversed by atipamezole.
Conclusion.
Our study demonstrated that Dex protected lung alveolar epithelial cells from apoptotic injury, cell cycle arrest, and loss of cell adhesion induced by H2O2 through enhancing the cell survival and proliferation.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Cui, Jian& Zhao, Hailin& Wang, Chunyan& Sun, James J.& Lu, Kaizhi& Ma, Daqing. 2015. Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075599
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Cui, Jian…[et al.]. Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075599
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Cui, Jian& Zhao, Hailin& Wang, Chunyan& Sun, James J.& Lu, Kaizhi& Ma, Daqing. Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075599
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1075599
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر