Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro
Joint Authors
Zhao, Hailin
Cui, Jian
Wang, Chunyan
Sun, James J.
Lu, Kaizhi
Ma, Daqing
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-03-08
Country of Publication
Egypt
No. of Pages
11
Main Subjects
Abstract EN
Background.
Oxidative stress plays a pivotal role in the lung injuries of critical ill patients.
This study investigates the protection conferred by α2 adrenoceptor agonist dexmedetomidine (Dex) from lung alveolar epithelial cell injury induced by hydrogen peroxide (H2O2) and the underlying mechanisms.
Methods.
The lung alveolar epithelial cell line, A549, was cultured and then treated with 500 μM H2O2 with or without Dex (1 nM) or Dex in combination with atipamezole (10 nM), an antagonist of α2 receptors.
Their effect on mitochondrial membrane potential (Δψm), reactive oxygen species (ROS), and the cell cycle was assessed by flow cytometry.
Cleaved-caspases 3 and 9, BAX, Bcl-2, phospho-mTOR (p-mTOR), ERK1/2, and E-cadherin expression were also determined with immunocytochemistry.
Results.
Upregulation of cleaved-caspases 3 and 9 and BAX and downregulation of Bcl-2, p-mTOR, and E-cadherin were found following H2O2 treatment, and all of these were reversed by Dex.
Dex also prevented the ROS generation, cytochrome C release, and cell cycle arrest induced by H2O2.
The effects of Dex were partially reversed by atipamezole.
Conclusion.
Our study demonstrated that Dex protected lung alveolar epithelial cells from apoptotic injury, cell cycle arrest, and loss of cell adhesion induced by H2O2 through enhancing the cell survival and proliferation.
American Psychological Association (APA)
Cui, Jian& Zhao, Hailin& Wang, Chunyan& Sun, James J.& Lu, Kaizhi& Ma, Daqing. 2015. Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075599
Modern Language Association (MLA)
Cui, Jian…[et al.]. Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075599
American Medical Association (AMA)
Cui, Jian& Zhao, Hailin& Wang, Chunyan& Sun, James J.& Lu, Kaizhi& Ma, Daqing. Dexmedetomidine Attenuates Oxidative Stress Induced Lung Alveolar Epithelial Cell Apoptosis In Vitro. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075599
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075599