Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells
المؤلفون المشاركون
Lu, Jianping
Li, Xinxiu
Chen, Zhaohong
Zeng, Caihong
Liu, Zhihong
Wang, Ya-Ping
Zhang, Mingchao
Chen, Huimei
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-10-20
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
MUTYH is a DNA repair enzyme that initiates a base excision repair (BER) by recognizing and removing 8-Oxoguanine (8-oxoG) and its paired adenine.
We demonstrated that both TGF-β1 and H2O2 treatment led to an increased 8-oxoG in cultured human proximal tubule epithelial (HK-2) cells, while the former induced epithelial-mesenchymal transition and the latter caused cell apoptosis.
Without stimulation, HK-2 cells showed MUTYH expression in mitochondria.
TGF-β1 triggered a transient upregulation of mitochondrial MUTYH and induced the expression of nuclear isoforms, while H2O2 showed no role on MUTYH expression.
Ureteral obstruction (UUO) mice exhibited high 8-oxoG reactivity with tubulointerstitial lesions.
After obstruction, the MUTYH expression was increased only in tubules at day 3 and decreased with obvious tubular atrophy at day 10.
Particularly, MUTYH was primarily located in normal tubular cytoplasm with a dominant mitochondrial form.
A few cells with nuclear MUTYH expression were observed in the fibrotic interstitium.
We confirmed that increased MUTYH expression was upregulated and positively correlated with the severity of kidney fibrosis.
Thus, renal fibrosis caused a cell-type-specific and time-dependent response of oxidative DNA repairs, even within the same tissues.
It suggests that intervention of MUTYH might be effective for therapies.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Jianping& Li, Xinxiu& Zhang, Mingchao& Chen, Zhaohong& Wang, Ya-Ping& Zeng, Caihong…[et al.]. 2015. Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075734
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Jianping…[et al.]. Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075734
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Jianping& Li, Xinxiu& Zhang, Mingchao& Chen, Zhaohong& Wang, Ya-Ping& Zeng, Caihong…[et al.]. Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075734
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1075734
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر