Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells
Joint Authors
Lu, Jianping
Li, Xinxiu
Chen, Zhaohong
Zeng, Caihong
Liu, Zhihong
Wang, Ya-Ping
Zhang, Mingchao
Chen, Huimei
Source
Oxidative Medicine and Cellular Longevity
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-10-20
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
MUTYH is a DNA repair enzyme that initiates a base excision repair (BER) by recognizing and removing 8-Oxoguanine (8-oxoG) and its paired adenine.
We demonstrated that both TGF-β1 and H2O2 treatment led to an increased 8-oxoG in cultured human proximal tubule epithelial (HK-2) cells, while the former induced epithelial-mesenchymal transition and the latter caused cell apoptosis.
Without stimulation, HK-2 cells showed MUTYH expression in mitochondria.
TGF-β1 triggered a transient upregulation of mitochondrial MUTYH and induced the expression of nuclear isoforms, while H2O2 showed no role on MUTYH expression.
Ureteral obstruction (UUO) mice exhibited high 8-oxoG reactivity with tubulointerstitial lesions.
After obstruction, the MUTYH expression was increased only in tubules at day 3 and decreased with obvious tubular atrophy at day 10.
Particularly, MUTYH was primarily located in normal tubular cytoplasm with a dominant mitochondrial form.
A few cells with nuclear MUTYH expression were observed in the fibrotic interstitium.
We confirmed that increased MUTYH expression was upregulated and positively correlated with the severity of kidney fibrosis.
Thus, renal fibrosis caused a cell-type-specific and time-dependent response of oxidative DNA repairs, even within the same tissues.
It suggests that intervention of MUTYH might be effective for therapies.
American Psychological Association (APA)
Lu, Jianping& Li, Xinxiu& Zhang, Mingchao& Chen, Zhaohong& Wang, Ya-Ping& Zeng, Caihong…[et al.]. 2015. Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075734
Modern Language Association (MLA)
Lu, Jianping…[et al.]. Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-9.
https://search.emarefa.net/detail/BIM-1075734
American Medical Association (AMA)
Lu, Jianping& Li, Xinxiu& Zhang, Mingchao& Chen, Zhaohong& Wang, Ya-Ping& Zeng, Caihong…[et al.]. Regulation of MUTYH, a DNA Repair Enzyme, in Renal Proximal Tubular Epithelial Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-9.
https://search.emarefa.net/detail/BIM-1075734
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1075734