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OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells
المؤلفون المشاركون
Liu, Zhong-Jie
Zhao, Wei
Zhang, Qing-Guo
Li, Le
Jiang, Shan
Xu, Shi-Yuan
Lai, Lu-ying
المصدر
Oxidative Medicine and Cellular Longevity
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-11، 11ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-06-16
دولة النشر
مصر
عدد الصفحات
11
التخصصات الرئيسية
الملخص EN
Hyperglycemia can inhibit expression of the 8-oxoG-DNA glycosylase (OGG1) which is one of the key repair enzymes for DNA oxidative damage.
The effect of hyperglycemia on OGG1 expression in response to local anesthetics-induced DNA damage is unknown.
This study was designed to determine whether high glucose inhibits OGG1 expression and aggravates bupivacaine-induced DNA damage via reactive oxygen species (ROS).
SH-SY5Y cells were cultured with or without 50 mM glucose for 8 days before they were treated with 1.5 mM bupivacaine for 24 h.
OGG1 expression was measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot.
ROS was estimated using the redox-sensitive fluorescent dye DCFH-DA.
DNA damage was investigated with immunostaining for 8-oxodG and comet assays.
OGG1 expression was inhibited in cells exposed to high glucose with concomitant increase in ROS production and more severe DNA damage as compared to control culture conditions, and these changes were further exacerbated by bupivacaine.
Treatment with the antioxidant N-acetyl-L-cysteine (NAC) prevented high glucose and bupivacaine mediated increase in ROS production and restored functional expression of OGG1, which lead to attenuated high glucose-mediated exacerbation of bupivacaine neurotoxicity.
Our findings indicate that subjects with diabetes may experience more detrimental effects following bupivacaine use.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Zhong-Jie& Zhao, Wei& Zhang, Qing-Guo& Li, Le& Lai, Lu-ying& Jiang, Shan…[et al.]. 2015. OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075735
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Zhong-Jie…[et al.]. OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075735
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Zhong-Jie& Zhao, Wei& Zhang, Qing-Guo& Li, Le& Lai, Lu-ying& Jiang, Shan…[et al.]. OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075735
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1075735
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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