OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells

Joint Authors

Liu, Zhong-Jie
Zhao, Wei
Zhang, Qing-Guo
Li, Le
Jiang, Shan
Xu, Shi-Yuan
Lai, Lu-ying

Source

Oxidative Medicine and Cellular Longevity

Issue

Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-11, 11 p.

Publisher

Hindawi Publishing Corporation

Publication Date

2015-06-16

Country of Publication

Egypt

No. of Pages

11

Main Subjects

Biology

Abstract EN

Hyperglycemia can inhibit expression of the 8-oxoG-DNA glycosylase (OGG1) which is one of the key repair enzymes for DNA oxidative damage.

The effect of hyperglycemia on OGG1 expression in response to local anesthetics-induced DNA damage is unknown.

This study was designed to determine whether high glucose inhibits OGG1 expression and aggravates bupivacaine-induced DNA damage via reactive oxygen species (ROS).

SH-SY5Y cells were cultured with or without 50 mM glucose for 8 days before they were treated with 1.5 mM bupivacaine for 24 h.

OGG1 expression was measured by quantitative real-time polymerase chain reaction (qRT-PCR) and western blot.

ROS was estimated using the redox-sensitive fluorescent dye DCFH-DA.

DNA damage was investigated with immunostaining for 8-oxodG and comet assays.

OGG1 expression was inhibited in cells exposed to high glucose with concomitant increase in ROS production and more severe DNA damage as compared to control culture conditions, and these changes were further exacerbated by bupivacaine.

Treatment with the antioxidant N-acetyl-L-cysteine (NAC) prevented high glucose and bupivacaine mediated increase in ROS production and restored functional expression of OGG1, which lead to attenuated high glucose-mediated exacerbation of bupivacaine neurotoxicity.

Our findings indicate that subjects with diabetes may experience more detrimental effects following bupivacaine use.

American Psychological Association (APA)

Liu, Zhong-Jie& Zhao, Wei& Zhang, Qing-Guo& Li, Le& Lai, Lu-ying& Jiang, Shan…[et al.]. 2015. OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity،Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075735

Modern Language Association (MLA)

Liu, Zhong-Jie…[et al.]. OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity No. 2015 (2015), pp.1-11.
https://search.emarefa.net/detail/BIM-1075735

American Medical Association (AMA)

Liu, Zhong-Jie& Zhao, Wei& Zhang, Qing-Guo& Li, Le& Lai, Lu-ying& Jiang, Shan…[et al.]. OGG1 Involvement in High Glucose-Mediated Enhancement of Bupivacaine-Induced Oxidative DNA Damage in SH-SY5Y Cells. Oxidative Medicine and Cellular Longevity. 2015. Vol. 2015, no. 2015, pp.1-11.
https://search.emarefa.net/detail/BIM-1075735

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references

Record ID

BIM-1075735