CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression
المؤلفون المشاركون
Tian, Feng
Ji, Xiang-lu
Xiao, Wan-an
Wang, Bin
Wang, Fei
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-02-16
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
CXC chemokines are essential for osteogenic differentiation of bone mesenchymal stem cells (BMSCs) for use in bone tissue engineering and regenerative medicine in clinical settings.
However, an accurate understanding of the underlying mechanisms is still needed.
In this study, we analyzed the effects of CXC chemokine ligand-13 (CXCL13) on osteogenic differentiation of rat BMSCs and initiated a preliminary discussion on possible mechanisms.
BMSCs were isolated from bone marrow of rat and incubated with CXCL13 recombinant protein in differentiation medium.
The main osteogenesis indexes were alkaline phosphatase (ALP) activity and calcium nodes.
Expression of Runx2 and CXCR5 was determined using western blot, while miRNAs were determined with quantitative-RT-PCR.
Si-CXCR5 was transfected into MSCs to silence CXCR5.
A miRNA-23a mimic was transfected into BMSCs for overexpression of miRNA-23a.
Recombinant CXCL13 induced ALP activity, deposition of calcium salts, and formation of calcium nodes, and it also increased expression of Runx2.
The expression of recombinant CXCL13 suppressed expression of miRNA-23a.
Overexpression of miR-23a reversed CXCL13 induced-osteogenic differentiation of BMSCs and expression of Runx2.
Recombinant CXCL13 attenuated the interaction of miRNA-23a with the Runx2 3′UTR.
Silencing of CXCR5 abrogated recombinant CXCL13-induced downregulation of miRNA-23a expression.
In summary, CXCL13 promotes osteogenic differentiation of BMSCs by inhibiting miR-23a expression.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Tian, Feng& Ji, Xiang-lu& Xiao, Wan-an& Wang, Bin& Wang, Fei. 2015. CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression. Stem Cells International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076304
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Tian, Feng…[et al.]. CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression. Stem Cells International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1076304
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Tian, Feng& Ji, Xiang-lu& Xiao, Wan-an& Wang, Bin& Wang, Fei. CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076304
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076304
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر