CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression
Joint Authors
Tian, Feng
Ji, Xiang-lu
Xiao, Wan-an
Wang, Bin
Wang, Fei
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-02-16
Country of Publication
Egypt
No. of Pages
8
Abstract EN
CXC chemokines are essential for osteogenic differentiation of bone mesenchymal stem cells (BMSCs) for use in bone tissue engineering and regenerative medicine in clinical settings.
However, an accurate understanding of the underlying mechanisms is still needed.
In this study, we analyzed the effects of CXC chemokine ligand-13 (CXCL13) on osteogenic differentiation of rat BMSCs and initiated a preliminary discussion on possible mechanisms.
BMSCs were isolated from bone marrow of rat and incubated with CXCL13 recombinant protein in differentiation medium.
The main osteogenesis indexes were alkaline phosphatase (ALP) activity and calcium nodes.
Expression of Runx2 and CXCR5 was determined using western blot, while miRNAs were determined with quantitative-RT-PCR.
Si-CXCR5 was transfected into MSCs to silence CXCR5.
A miRNA-23a mimic was transfected into BMSCs for overexpression of miRNA-23a.
Recombinant CXCL13 induced ALP activity, deposition of calcium salts, and formation of calcium nodes, and it also increased expression of Runx2.
The expression of recombinant CXCL13 suppressed expression of miRNA-23a.
Overexpression of miR-23a reversed CXCL13 induced-osteogenic differentiation of BMSCs and expression of Runx2.
Recombinant CXCL13 attenuated the interaction of miRNA-23a with the Runx2 3′UTR.
Silencing of CXCR5 abrogated recombinant CXCL13-induced downregulation of miRNA-23a expression.
In summary, CXCL13 promotes osteogenic differentiation of BMSCs by inhibiting miR-23a expression.
American Psychological Association (APA)
Tian, Feng& Ji, Xiang-lu& Xiao, Wan-an& Wang, Bin& Wang, Fei. 2015. CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression. Stem Cells International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076304
Modern Language Association (MLA)
Tian, Feng…[et al.]. CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression. Stem Cells International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1076304
American Medical Association (AMA)
Tian, Feng& Ji, Xiang-lu& Xiao, Wan-an& Wang, Bin& Wang, Fei. CXCL13 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Inhibiting miR-23a Expression. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076304
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076304