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A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy
المؤلفون المشاركون
Luo, Yumei
Zhu, Detu
Lam, Dang Hoang
Huang, Juan
Tang, Yi
Luo, Xitu
Wang, Shu
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-05
دولة النشر
مصر
عدد الصفحات
8
الملخص EN
Recent progress in neural stem cell- (NSC-) based tumor-targeted gene therapy showed that NSC vectors expressing an artificially engineered viral fusogenic protein, VSV-G H162R, could cause tumor cell death specifically under acidic tumor microenvironment by syncytia formation; however, the killing efficiency still had much room to improve.
In the view that coexpression of another antitumoral gene with VSV-G can augment the bystander effect, a synthetic regulatory system that triggers transgene expression in a cell fusion-inducible manner has been proposed.
Here we have developed a double-switch cell fusion-inducible transgene expression system (DoFIT) to drive transgene expression upon VSV-G-mediated NSC-glioma cell fusion.
In this binary system, transgene expression is coregulated by a glioma-specific promoter and targeting sequences of a microRNA (miR) that is highly expressed in NSCs but lowly expressed in glioma cells.
Thus, transgene expression is “switched off” by the miR in NSC vectors, but after cell fusion with glioma cells, the miR is diluted and loses its suppressive effect.
Meanwhile, in the syncytia, transgene expression is “switched on” by the glioma-specific promoter.
Our in vitro and in vivo experimental data show that DoFIT successfully abolishes luciferase reporter gene expression in NSC vectors but activates it specifically after VSV-G-mediated NSC-glioma cell fusion.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Luo, Yumei& Zhu, Detu& Lam, Dang Hoang& Huang, Juan& Tang, Yi& Luo, Xitu…[et al.]. 2015. A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy. Stem Cells International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076315
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Luo, Yumei…[et al.]. A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy. Stem Cells International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1076315
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Luo, Yumei& Zhu, Detu& Lam, Dang Hoang& Huang, Juan& Tang, Yi& Luo, Xitu…[et al.]. A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076315
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076315
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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