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A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy
Joint Authors
Luo, Yumei
Zhu, Detu
Lam, Dang Hoang
Huang, Juan
Tang, Yi
Luo, Xitu
Wang, Shu
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-05
Country of Publication
Egypt
No. of Pages
8
Abstract EN
Recent progress in neural stem cell- (NSC-) based tumor-targeted gene therapy showed that NSC vectors expressing an artificially engineered viral fusogenic protein, VSV-G H162R, could cause tumor cell death specifically under acidic tumor microenvironment by syncytia formation; however, the killing efficiency still had much room to improve.
In the view that coexpression of another antitumoral gene with VSV-G can augment the bystander effect, a synthetic regulatory system that triggers transgene expression in a cell fusion-inducible manner has been proposed.
Here we have developed a double-switch cell fusion-inducible transgene expression system (DoFIT) to drive transgene expression upon VSV-G-mediated NSC-glioma cell fusion.
In this binary system, transgene expression is coregulated by a glioma-specific promoter and targeting sequences of a microRNA (miR) that is highly expressed in NSCs but lowly expressed in glioma cells.
Thus, transgene expression is “switched off” by the miR in NSC vectors, but after cell fusion with glioma cells, the miR is diluted and loses its suppressive effect.
Meanwhile, in the syncytia, transgene expression is “switched on” by the glioma-specific promoter.
Our in vitro and in vivo experimental data show that DoFIT successfully abolishes luciferase reporter gene expression in NSC vectors but activates it specifically after VSV-G-mediated NSC-glioma cell fusion.
American Psychological Association (APA)
Luo, Yumei& Zhu, Detu& Lam, Dang Hoang& Huang, Juan& Tang, Yi& Luo, Xitu…[et al.]. 2015. A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy. Stem Cells International،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076315
Modern Language Association (MLA)
Luo, Yumei…[et al.]. A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy. Stem Cells International No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1076315
American Medical Association (AMA)
Luo, Yumei& Zhu, Detu& Lam, Dang Hoang& Huang, Juan& Tang, Yi& Luo, Xitu…[et al.]. A Double-Switch Cell Fusion-Inducible Transgene Expression System for Neural Stem Cell-Based Antiglioma Gene Therapy. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076315
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076315