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Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction
المؤلفون المشاركون
Kang, Kai
Ma, Ruilian
Cai, Wenfeng
Huang, Wei
Paul, Christian
Liang, Jialiang
Wang, Yuhua
Zhao, Tiejun
Kim, Ha Won
Xu, Meifeng
Millard, Ronald W.
Wen, Zhili
Wang, Yigang
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-14، 14ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-05-05
دولة النشر
مصر
عدد الصفحات
14
الملخص EN
Background and Objective.
Exosomes secreted from mesenchymal stem cells (MSC) have demonstrated cardioprotective effects.
This study examined the role of exosomes derived from MSC overexpressing CXCR4 for recovery of cardiac functions after myocardial infarction (MI).
Methods.
In vitro, exosomes from MSC transduced with lentiviral CXCR4 (ExoCR4) encoding a silencing sequence or null vector were isolated and characterized by transmission electron microscopy and dynamic light scattering.
Gene expression was then analyzed by qPCR and Western blotting.
Cytoprotective effects on cardiomyocytes were evaluated and effects of exosomes on angiogenesis analyzed.
In vivo, an exosome-pretreated MSC-sheet was implanted into a region of scarred myocardium in a rat MI model.
Angiogenesis, infarct size, and cardiac functions were then analyzed.
Results.
In vitro, ExoCR4 significantly upregulated IGF-1α and pAkt levels and downregulated active caspase 3 level in cardiomyocytes.
ExoCR4 also enhanced VEGF expression and vessel formation.
However, effects of ExoCR4 were abolished by an Akt inhibitor or CXCR4 knockdown.
In vivo, ExoCR4 treated MSC-sheet implantation promoted cardiac functional restoration by increasing angiogenesis, reducing infarct size, and improving cardiac remodeling.
Conclusions.
This study reveals a novel role of exosomes derived from MSCCR4 and highlights a new mechanism of intercellular mediation of stem cells for MI treatment.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Kang, Kai& Ma, Ruilian& Cai, Wenfeng& Huang, Wei& Paul, Christian& Liang, Jialiang…[et al.]. 2015. Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction. Stem Cells International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076322
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Kang, Kai…[et al.]. Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction. Stem Cells International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1076322
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Kang, Kai& Ma, Ruilian& Cai, Wenfeng& Huang, Wei& Paul, Christian& Liang, Jialiang…[et al.]. Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076322
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076322
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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