Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction
Joint Authors
Kang, Kai
Ma, Ruilian
Cai, Wenfeng
Huang, Wei
Paul, Christian
Liang, Jialiang
Wang, Yuhua
Zhao, Tiejun
Kim, Ha Won
Xu, Meifeng
Millard, Ronald W.
Wen, Zhili
Wang, Yigang
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-14, 14 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-05-05
Country of Publication
Egypt
No. of Pages
14
Abstract EN
Background and Objective.
Exosomes secreted from mesenchymal stem cells (MSC) have demonstrated cardioprotective effects.
This study examined the role of exosomes derived from MSC overexpressing CXCR4 for recovery of cardiac functions after myocardial infarction (MI).
Methods.
In vitro, exosomes from MSC transduced with lentiviral CXCR4 (ExoCR4) encoding a silencing sequence or null vector were isolated and characterized by transmission electron microscopy and dynamic light scattering.
Gene expression was then analyzed by qPCR and Western blotting.
Cytoprotective effects on cardiomyocytes were evaluated and effects of exosomes on angiogenesis analyzed.
In vivo, an exosome-pretreated MSC-sheet was implanted into a region of scarred myocardium in a rat MI model.
Angiogenesis, infarct size, and cardiac functions were then analyzed.
Results.
In vitro, ExoCR4 significantly upregulated IGF-1α and pAkt levels and downregulated active caspase 3 level in cardiomyocytes.
ExoCR4 also enhanced VEGF expression and vessel formation.
However, effects of ExoCR4 were abolished by an Akt inhibitor or CXCR4 knockdown.
In vivo, ExoCR4 treated MSC-sheet implantation promoted cardiac functional restoration by increasing angiogenesis, reducing infarct size, and improving cardiac remodeling.
Conclusions.
This study reveals a novel role of exosomes derived from MSCCR4 and highlights a new mechanism of intercellular mediation of stem cells for MI treatment.
American Psychological Association (APA)
Kang, Kai& Ma, Ruilian& Cai, Wenfeng& Huang, Wei& Paul, Christian& Liang, Jialiang…[et al.]. 2015. Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction. Stem Cells International،Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076322
Modern Language Association (MLA)
Kang, Kai…[et al.]. Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction. Stem Cells International No. 2015 (2015), pp.1-14.
https://search.emarefa.net/detail/BIM-1076322
American Medical Association (AMA)
Kang, Kai& Ma, Ruilian& Cai, Wenfeng& Huang, Wei& Paul, Christian& Liang, Jialiang…[et al.]. Exosomes Secreted from CXCR4 Overexpressing Mesenchymal Stem Cells Promote Cardioprotection via Akt Signaling Pathway following Myocardial Infarction. Stem Cells International. 2015. Vol. 2015, no. 2015, pp.1-14.
https://search.emarefa.net/detail/BIM-1076322
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076322