An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture
المؤلفون المشاركون
Shekhawat, Mahipal S.
Manokari, M.
Ravindran, C. P.
المصدر
العدد
المجلد 2015، العدد 2015 (31 ديسمبر/كانون الأول 2015)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2015-07-26
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
A procedure for rapid clonal propagation of Passiflora edulis Sims.
f.
flavicarpa Deg.
(Passifloraceae) has been developed in this study.
Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium.
The addition of 2.0 mgL−1 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots ( 8.21 ± 1.13 ) primordial from the nodal meristems.
Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1 of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots in vitro with maximum numbers of shoots ( 25.73 ± 0.06 ) within four weeks of incubation.
Shoots were rooted best ( 7.13 ± 0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1 indole-3 butyric acid (IBA).
All in vitro regenerated shoots were rooted by ex vitro method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1 IBA.
The plantlets were hardened in the greenhouse for 4-5 weeks.
The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Shekhawat, Mahipal S.& Manokari, M.& Ravindran, C. P.. 2015. An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture. Scientifica،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076453
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Shekhawat, Mahipal S.…[et al.]. An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture. Scientifica No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1076453
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Shekhawat, Mahipal S.& Manokari, M.& Ravindran, C. P.. An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture. Scientifica. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076453
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1076453
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر