An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture
Joint Authors
Shekhawat, Mahipal S.
Manokari, M.
Ravindran, C. P.
Source
Issue
Vol. 2015, Issue 2015 (31 Dec. 2015), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2015-07-26
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
A procedure for rapid clonal propagation of Passiflora edulis Sims.
f.
flavicarpa Deg.
(Passifloraceae) has been developed in this study.
Nodal explants were sterilized with 0.1% HgCl2 and inoculated on Murashige and Skoog (MS) basal medium.
The addition of 2.0 mgL−1 6-benzylaminopurine (BAP) to MS medium caused an extensive proliferation of multiple shoots ( 8.21 ± 1.13 ) primordial from the nodal meristems.
Subculturing of these multiple shoots on the MS medium augmented with 1.0 mgL−1 of each BAP and Kinetin (Kin) was successful for the multiplication of the shoots in vitro with maximum numbers of shoots ( 25.73 ± 0.06 ) within four weeks of incubation.
Shoots were rooted best ( 7.13 ± 0.56 roots/shoots) on half strength MS medium supplemented with 2.0 mgL−1 indole-3 butyric acid (IBA).
All in vitro regenerated shoots were rooted by ex vitro method, and this has achieved 6-7 roots per shoot by pulsing of cut ends of the shoots using 200 as well as 300 mgL−1 IBA.
The plantlets were hardened in the greenhouse for 4-5 weeks.
The hardened plantlets were shifted to manure containing nursery polybags after five weeks and then transferred to a sand bed for another four weeks for acclimatization before field planting with 88% survival rate.
American Psychological Association (APA)
Shekhawat, Mahipal S.& Manokari, M.& Ravindran, C. P.. 2015. An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture. Scientifica،Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076453
Modern Language Association (MLA)
Shekhawat, Mahipal S.…[et al.]. An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture. Scientifica No. 2015 (2015), pp.1-8.
https://search.emarefa.net/detail/BIM-1076453
American Medical Association (AMA)
Shekhawat, Mahipal S.& Manokari, M.& Ravindran, C. P.. An Improved Micropropagation Protocol by Ex Vitro Rooting of Passiflora edulis Sims. f. flavicarpa Deg. through Nodal Segment Culture. Scientifica. 2015. Vol. 2015, no. 2015, pp.1-8.
https://search.emarefa.net/detail/BIM-1076453
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1076453