The role of natural of antisensetranscription HAGLR,LCMT1AS, NAV2AS5,TSIX in breast cancer

الملخص EN

Backgraound: lncRNAs (longnon-codingRNAs), has avarious important molecular and cellular functions and natural antisense transcripts (NATs), complementary to protein-coding or non-coding.

RNA sequences are important regulators of gene expression drew a great attention recent years to uncover their importance for diagnostic, prognostic and therapeutic purposes; and their dysfunctions leads to diseases including cancer.

Aims:The aim of this study was to investigate important roles of lncNATs TSIX, HAGLR, LMCT1AS and NAV2AS5 genes in several tumorigenic processes in breast cancer.

Materials andMethods: In this study we used ATCC normal cell lines (CRL4010,CRL8798) and cancer cell lines (MCF7,MDA-MB231,CRL2329) that subjected.

To examine through RNA isolation,cDNAconversion, semiquantitative (by agarose gel and ImageJ program) and quantitative RT-PCR for gene expression analyses.

Results:Our results have been shown that TSIX, HAGLR, LMCT1AS and NAV2AS5 genes have differential expression pattern in both normal and breast cancer cell lines.

Conclution: from the data of our results we concluded that these genes have important role in biological procesess of breast cancer in addition their impotnace in treatment and therapeutic purpus.