Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in stevia rebaudiana (bertoni)‎

المؤلفون المشاركون

Bakhit, Makhluf Muhammad Mahmud
Abd al-Sabur, Muhammad Siraj al-Din
Shihab, Ilham
al-Aqqad, T. A. M.
al-Shaykh, A. A.

المصدر

Annals of Agricultural Science, Moshtohor

الناشر

جامعة بنها كلية الزراعة

تاريخ النشر

2018-04-30

دولة النشر

مصر

عدد الصفحات

10

التخصصات الرئيسية

العلوم الزراعية

الموضوعات

• التنوع الحيوي

الملخص الإنجليزي

Chromosome number was investigated in a number of strains of Stevia rebaudiana.

The chromosomes were analyzed during mitosis and different stages of meiosis.

All stevia strains were found to had 2n = 22.

The number of bivalents at diakinesis was 11 for all the studied strains.

Analysis of SDS-PAGE of soluble protein revealed complete similarity (100% ) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band.

Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships.

The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR).

A total of 231 clear bands were generated, out of which 34 (14.72 % ) were unique bands.

The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer.

نوع البيانات

أوراق مؤتمرات

رقم السجل

BIM-1093713

• APA
• MLA
• AMA