Role of cytogenetic studies, protein electrophoresis and ISSR markers in assessment of genetic diversity in stevia rebaudiana (bertoni)‎

Joint Authors

Bakhit, Makhluf Muhammad Mahmud
Abd al-Sabur, Muhammad Siraj al-Din
Shihab, Ilham
al-Aqqad, T. A. M.
al-Shaykh, A. A.

Source

Annals of Agricultural Science, Moshtohor

Publisher

Banha University Faculty of Agriculture

Publication Date

2018-04-30

Country of Publication

Egypt

No. of Pages

10

Main Subjects

Agriculture

Topics

• Biological diversity

English Abstract

Chromosome number was investigated in a number of strains of Stevia rebaudiana.

The chromosomes were analyzed during mitosis and different stages of meiosis.

All stevia strains were found to had 2n = 22.

The number of bivalents at diakinesis was 11 for all the studied strains.

Analysis of SDS-PAGE of soluble protein revealed complete similarity (100% ) among the six strains of Stevia while two strains (broad and narrow leaves) are different in one band.

Eight genotypes were assayed by inter-simple sequence repeat (ISSR) markers to differentiate and explore their genetic relationships.

The level of polymorphism detected among the eight strains of stevia was 100.00% using inter simple sequence repeat (ISSR).

A total of 231 clear bands were generated, out of which 34 (14.72 % ) were unique bands.

The total number of markers varied from 1 to 12 with a mean of 5.67 markers per primer.

Data Type

Conference Papers

Record ID

BIM-1093713

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