Studying the optimum conditions of hygromycin B production and detect their toxicity

المؤلفون المشاركون

Abd al-Munim, Zahra
Jabbar, Rabah
al-Shaybani, Abd al-Wahid Baqir

المصدر

Journal of Babylon University : Journal of Applied and Pure Sciences

العدد

المجلد 26، العدد 2 (28 فبراير/شباط 2018)، ص ص. 119-130، 12ص.

الناشر

جامعة بابل

تاريخ النشر

2018-02-28

دولة النشر

العراق

عدد الصفحات

12

التخصصات الرئيسية

الأحياء

الملخص EN

Hygromycin B was extracted with ethyl acetate, which separates organic phase from aqueous phase in the broth culture filtrate, only the aqueous phase showed significant antimicrobial activity by using agar well diffusion technique.

At a concentration of 25mg/ml (as crude extract), this phase excreted its activity against the test microorganisms which include; one G(+) bacteria (Staphylococcus aureus), five G(–) bacteria (Pseudomonas aeruginosa , Proteus mirabilis, Escherichia coli , Klebsiella pneumoniae, Salmonella typhi) and one yeast (Saccharomyces cerevisiae).

After detecting the aminoglycoside hygromycin B by the Thin Layer Chromatography (TLC) method to ensure presence of the antibiotic, same flow rate (Rf) value (0.357) as that of the standard hygromycin B was obtained.

Results of the optimization conditions showed that the highest antimicrobial activity of hygromycin B was obtained at a medium pH of 8 and incubation temperature of 35°C for 10 days.

When the toxicity of hygromycin B crude extract under such conditions was examined on mice liver, a mild effects were appeared.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Abd al-Munim, Zahra& Jabbar, Rabah& al-Shaybani, Abd al-Wahid Baqir. 2018. Studying the optimum conditions of hygromycin B production and detect their toxicity. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 26, no. 2, pp.119-130.
https://search.emarefa.net/detail/BIM-1094071

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Abd al-Munim, Zahra…[et al.]. Studying the optimum conditions of hygromycin B production and detect their toxicity. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 26, no. 2 (2018), pp.119-130.
https://search.emarefa.net/detail/BIM-1094071

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Abd al-Munim, Zahra& Jabbar, Rabah& al-Shaybani, Abd al-Wahid Baqir. Studying the optimum conditions of hygromycin B production and detect their toxicity. Journal of Babylon University : Journal of Applied and Pure Sciences. 2018. Vol. 26, no. 2, pp.119-130.
https://search.emarefa.net/detail/BIM-1094071

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references : p. 129-130

رقم السجل

BIM-1094071