Studying the optimum conditions of hygromycin B production and detect their toxicity
Joint Authors
Abd al-Munim, Zahra
Jabbar, Rabah
al-Shaybani, Abd al-Wahid Baqir
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 26, Issue 2 (28 Feb. 2018), pp.119-130, 12 p.
Publisher
Publication Date
2018-02-28
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
Hygromycin B was extracted with ethyl acetate, which separates organic phase from aqueous phase in the broth culture filtrate, only the aqueous phase showed significant antimicrobial activity by using agar well diffusion technique.
At a concentration of 25mg/ml (as crude extract), this phase excreted its activity against the test microorganisms which include; one G(+) bacteria (Staphylococcus aureus), five G(–) bacteria (Pseudomonas aeruginosa , Proteus mirabilis, Escherichia coli , Klebsiella pneumoniae, Salmonella typhi) and one yeast (Saccharomyces cerevisiae).
After detecting the aminoglycoside hygromycin B by the Thin Layer Chromatography (TLC) method to ensure presence of the antibiotic, same flow rate (Rf) value (0.357) as that of the standard hygromycin B was obtained.
Results of the optimization conditions showed that the highest antimicrobial activity of hygromycin B was obtained at a medium pH of 8 and incubation temperature of 35°C for 10 days.
When the toxicity of hygromycin B crude extract under such conditions was examined on mice liver, a mild effects were appeared.
American Psychological Association (APA)
Abd al-Munim, Zahra& Jabbar, Rabah& al-Shaybani, Abd al-Wahid Baqir. 2018. Studying the optimum conditions of hygromycin B production and detect their toxicity. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 26, no. 2, pp.119-130.
https://search.emarefa.net/detail/BIM-1094071
Modern Language Association (MLA)
Abd al-Munim, Zahra…[et al.]. Studying the optimum conditions of hygromycin B production and detect their toxicity. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 26, no. 2 (2018), pp.119-130.
https://search.emarefa.net/detail/BIM-1094071
American Medical Association (AMA)
Abd al-Munim, Zahra& Jabbar, Rabah& al-Shaybani, Abd al-Wahid Baqir. Studying the optimum conditions of hygromycin B production and detect their toxicity. Journal of Babylon University : Journal of Applied and Pure Sciences. 2018. Vol. 26, no. 2, pp.119-130.
https://search.emarefa.net/detail/BIM-1094071
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 129-130
Record ID
BIM-1094071