Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells
المؤلفون المشاركون
Skytt, D. M.
Toft-Kehler, A. K.
Brændstrup, C. T.
Cejvanovic, S.
Gurubaran, I. S.
Bergersen, L. H.
Kolko, M.
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-26
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
Glia-neuron partnership is important for inner retinal homeostasis and any disturbances may result in retinal ganglion cell (RGC) death.
Müller cells support RGCs with essential functions such as removing excess glutamate and providing energy sources.
The aim was to explore the impact of Müller cells on RGC survival.
To investigate the Müller cell/RGC interactions we developed a coculture model, in which primary Müller cells were grown in inserts on top of pure primary RGC cultures.
The impact of starvation and mitochondrial inhibition on the Müller cell ability to protect RGCs was studied.
Moreover, the ability of Müller cells to remove glutamate from the extracellular space was investigated.
RGC survival was evaluated by cell viability assays and glutamate uptake was assessed by kinetic uptake assays.
We demonstrated a significantly increased RGC survival in presence of untreated and prestarved Müller cells.
Additionally, prestarved Müller cells significantly increased RGC survival after mitochondrial inhibition.
Finally, we revealed a significantly increased ability to take up glutamate in starved Müller cells.
Overall, our study confirms essential roles of Müller cells in RGC survival.
We suggest that targeting Müller cell function could have potential for future treatment strategies to prevent blinding neurodegenerative retinal diseases.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Skytt, D. M.& Toft-Kehler, A. K.& Brændstrup, C. T.& Cejvanovic, S.& Gurubaran, I. S.& Bergersen, L. H.…[et al.]. 2016. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096824
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Skytt, D. M.…[et al.]. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1096824
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Skytt, D. M.& Toft-Kehler, A. K.& Brændstrup, C. T.& Cejvanovic, S.& Gurubaran, I. S.& Bergersen, L. H.…[et al.]. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096824
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1096824
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر