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Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells
Joint Authors
Skytt, D. M.
Toft-Kehler, A. K.
Brændstrup, C. T.
Cejvanovic, S.
Gurubaran, I. S.
Bergersen, L. H.
Kolko, M.
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-06-26
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
Glia-neuron partnership is important for inner retinal homeostasis and any disturbances may result in retinal ganglion cell (RGC) death.
Müller cells support RGCs with essential functions such as removing excess glutamate and providing energy sources.
The aim was to explore the impact of Müller cells on RGC survival.
To investigate the Müller cell/RGC interactions we developed a coculture model, in which primary Müller cells were grown in inserts on top of pure primary RGC cultures.
The impact of starvation and mitochondrial inhibition on the Müller cell ability to protect RGCs was studied.
Moreover, the ability of Müller cells to remove glutamate from the extracellular space was investigated.
RGC survival was evaluated by cell viability assays and glutamate uptake was assessed by kinetic uptake assays.
We demonstrated a significantly increased RGC survival in presence of untreated and prestarved Müller cells.
Additionally, prestarved Müller cells significantly increased RGC survival after mitochondrial inhibition.
Finally, we revealed a significantly increased ability to take up glutamate in starved Müller cells.
Overall, our study confirms essential roles of Müller cells in RGC survival.
We suggest that targeting Müller cell function could have potential for future treatment strategies to prevent blinding neurodegenerative retinal diseases.
American Psychological Association (APA)
Skytt, D. M.& Toft-Kehler, A. K.& Brændstrup, C. T.& Cejvanovic, S.& Gurubaran, I. S.& Bergersen, L. H.…[et al.]. 2016. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096824
Modern Language Association (MLA)
Skytt, D. M.…[et al.]. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1096824
American Medical Association (AMA)
Skytt, D. M.& Toft-Kehler, A. K.& Brændstrup, C. T.& Cejvanovic, S.& Gurubaran, I. S.& Bergersen, L. H.…[et al.]. Glia-Neuron Interactions in the Retina Can Be Studied in Cocultures of Müller Cells and Retinal Ganglion Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1096824
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096824