Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels
المؤلفون المشاركون
Wu, Haidong
Wang, Peng
Li, Yi
Wu, Manhui
Lin, Jiali
Huang, Zitong
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-6، 6ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-28
دولة النشر
مصر
عدد الصفحات
6
التخصصات الرئيسية
الملخص EN
Objective.
We investigated whether and how diazoxide can attenuate brain injury after cardiopulmonary resuscitation (CPR) by selective opening of mitochondrial ATP-sensitive potassium (mitoKATP) channels.
Methods.
Adult male Sprague-Dawley rats with induced cerebral ischemia (n=10 per group) received an intraperitoneal injection of 0.1% dimethyl sulfoxide (1 mL; vehicle group), diazoxide (10 mg/kg; DZ group), or diazoxide (10 mg/kg) plus 5-hydroxydecanoate (5 mg/kg; DZ + 5-HD group) 30 min after CPR.
The control group (sham group, n=5) underwent sham operation, without cardiac arrest.
Mitochondrial respiratory control rate (RCR) was determined.
Brain cell apoptosis was assessed using TUNEL staining.
Expression of Bcl-2, Bax, and protein kinase C epsilon (PKCε) in the cerebral cortex was determined by Western blotting and immunohistochemistry.
Results.
The neurological deficit scores (NDS) in the vehicle group decreased significantly at 24 h and 48 h after CPR.
Diazoxide significantly improved NDS and mitochondrial RCR after CPR at both time points; 5-HD cotreatment abolished these effects.
Diazoxide decreased TUNEL-positive cells following CPR, upregulated Bcl-2 and PKCε, downregulated Bax, and increased the Bcl-2/Bax ratio; 5-HD cotreatment reversed these effects.
Conclusions.
Diazoxide attenuates postresuscitation brain injury, protects mitochondrial function, inhibits brain cell apoptosis, and activates the PKC pathway by opening mitoKATP channels.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Wu, Haidong& Wang, Peng& Li, Yi& Wu, Manhui& Lin, Jiali& Huang, Zitong. 2016. Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1096837
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Wu, Haidong…[et al.]. Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1096837
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Wu, Haidong& Wang, Peng& Li, Yi& Wu, Manhui& Lin, Jiali& Huang, Zitong. Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1096837
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1096837
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر