Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels
Joint Authors
Wu, Haidong
Wang, Peng
Li, Yi
Wu, Manhui
Lin, Jiali
Huang, Zitong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-6, 6 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-28
Country of Publication
Egypt
No. of Pages
6
Main Subjects
Abstract EN
Objective.
We investigated whether and how diazoxide can attenuate brain injury after cardiopulmonary resuscitation (CPR) by selective opening of mitochondrial ATP-sensitive potassium (mitoKATP) channels.
Methods.
Adult male Sprague-Dawley rats with induced cerebral ischemia (n=10 per group) received an intraperitoneal injection of 0.1% dimethyl sulfoxide (1 mL; vehicle group), diazoxide (10 mg/kg; DZ group), or diazoxide (10 mg/kg) plus 5-hydroxydecanoate (5 mg/kg; DZ + 5-HD group) 30 min after CPR.
The control group (sham group, n=5) underwent sham operation, without cardiac arrest.
Mitochondrial respiratory control rate (RCR) was determined.
Brain cell apoptosis was assessed using TUNEL staining.
Expression of Bcl-2, Bax, and protein kinase C epsilon (PKCε) in the cerebral cortex was determined by Western blotting and immunohistochemistry.
Results.
The neurological deficit scores (NDS) in the vehicle group decreased significantly at 24 h and 48 h after CPR.
Diazoxide significantly improved NDS and mitochondrial RCR after CPR at both time points; 5-HD cotreatment abolished these effects.
Diazoxide decreased TUNEL-positive cells following CPR, upregulated Bcl-2 and PKCε, downregulated Bax, and increased the Bcl-2/Bax ratio; 5-HD cotreatment reversed these effects.
Conclusions.
Diazoxide attenuates postresuscitation brain injury, protects mitochondrial function, inhibits brain cell apoptosis, and activates the PKC pathway by opening mitoKATP channels.
American Psychological Association (APA)
Wu, Haidong& Wang, Peng& Li, Yi& Wu, Manhui& Lin, Jiali& Huang, Zitong. 2016. Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels. BioMed Research International،Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1096837
Modern Language Association (MLA)
Wu, Haidong…[et al.]. Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels. BioMed Research International No. 2016 (2016), pp.1-6.
https://search.emarefa.net/detail/BIM-1096837
American Medical Association (AMA)
Wu, Haidong& Wang, Peng& Li, Yi& Wu, Manhui& Lin, Jiali& Huang, Zitong. Diazoxide Attenuates Postresuscitation Brain Injury in a Rat Model of Asphyxial Cardiac Arrest by Opening Mitochondrial ATP-Sensitive Potassium Channels. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-6.
https://search.emarefa.net/detail/BIM-1096837
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1096837