PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells
المؤلفون المشاركون
Chen, Qiong
Lu, Xiao-Xiao
Cao, Lan-Yu
Chen, Xi
Xiao, Jian
Zou, Yong
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-16
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
PTEN plays an essential role in tumorigenesis and both its mutation and inactivation can influence proliferation, apoptosis, and cell cycle progression in tumor cells.
However, the precise role of PTEN in lung cancer cells has not been well studied.
To address this, we have generated lung adenocarcinoma A549 cells overexpressing wild-type or mutant PTEN as well as A549 cells expressing a siRNA directed toward endogenous PTEN.
Overexpression of wild-type PTEN profoundly inhibited cell proliferation, promoted cell apoptosis, caused cell cycle arrest at G1, downregulated p-AKT, and decreased expression of the telomerase protein hTERT.
In contrast, in cells expressing a PTEN directed siRNA, the opposite effects on cell proliferation, apoptosis, cell cycle arrest, p-AKT levels, and hTERT protein expression were observed.
A549 cells transfected with a PTEN mutant lacking phosphatase activity (PTEN-C124A) or an empty vector (null) did not show any effect.
Furthermore, using the PI3K/AKT pathway blocker LY294002, we confirmed that the PI3K/AKT pathway was involved in mediating these effects of PTEN.
Taken together, we have demonstrated that PTEN downregulates the PI3K/AKT/hTERT pathway, thereby suppressing the growth of lung adenocarcinoma cells.
Our study may provide evidence for a promising therapeutic target for the treatment of lung adenocarcinoma.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Xiao-Xiao& Cao, Lan-Yu& Chen, Xi& Xiao, Jian& Zou, Yong& Chen, Qiong. 2016. PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097078
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Xiao-Xiao…[et al.]. PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1097078
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Xiao-Xiao& Cao, Lan-Yu& Chen, Xi& Xiao, Jian& Zou, Yong& Chen, Qiong. PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097078
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1097078
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر