PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells
Joint Authors
Chen, Qiong
Lu, Xiao-Xiao
Cao, Lan-Yu
Chen, Xi
Xiao, Jian
Zou, Yong
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-16
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
PTEN plays an essential role in tumorigenesis and both its mutation and inactivation can influence proliferation, apoptosis, and cell cycle progression in tumor cells.
However, the precise role of PTEN in lung cancer cells has not been well studied.
To address this, we have generated lung adenocarcinoma A549 cells overexpressing wild-type or mutant PTEN as well as A549 cells expressing a siRNA directed toward endogenous PTEN.
Overexpression of wild-type PTEN profoundly inhibited cell proliferation, promoted cell apoptosis, caused cell cycle arrest at G1, downregulated p-AKT, and decreased expression of the telomerase protein hTERT.
In contrast, in cells expressing a PTEN directed siRNA, the opposite effects on cell proliferation, apoptosis, cell cycle arrest, p-AKT levels, and hTERT protein expression were observed.
A549 cells transfected with a PTEN mutant lacking phosphatase activity (PTEN-C124A) or an empty vector (null) did not show any effect.
Furthermore, using the PI3K/AKT pathway blocker LY294002, we confirmed that the PI3K/AKT pathway was involved in mediating these effects of PTEN.
Taken together, we have demonstrated that PTEN downregulates the PI3K/AKT/hTERT pathway, thereby suppressing the growth of lung adenocarcinoma cells.
Our study may provide evidence for a promising therapeutic target for the treatment of lung adenocarcinoma.
American Psychological Association (APA)
Lu, Xiao-Xiao& Cao, Lan-Yu& Chen, Xi& Xiao, Jian& Zou, Yong& Chen, Qiong. 2016. PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097078
Modern Language Association (MLA)
Lu, Xiao-Xiao…[et al.]. PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1097078
American Medical Association (AMA)
Lu, Xiao-Xiao& Cao, Lan-Yu& Chen, Xi& Xiao, Jian& Zou, Yong& Chen, Qiong. PTEN Inhibits Cell Proliferation, Promotes Cell Apoptosis, and Induces Cell Cycle Arrest via Downregulating the PI3KAKThTERT Pathway in Lung Adenocarcinoma A549 Cells. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1097078
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097078