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Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture
المؤلفون المشاركون
Zhu, Liguo
Feng, Minshan
Zhan, Jia-Wen
Zhang, Ping
Yu, Jie
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-10، 10ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-10-31
دولة النشر
مصر
عدد الصفحات
10
التخصصات الرئيسية
الملخص EN
The development of mechanically active culture systems helps in understanding of the role of mechanical stress in intervertebral disc (IVD) degeneration.
Motion segment cultures facilitate the application and control of mechanical loads.
The purpose of this study was to establish a culturing method for rabbit IVD motion segments to observe the effect of static load on the whole disc organ.
Segments were cultured in custom-made apparatuses under a constant, compressive load (3 kg) for 2 weeks.
Tissue integrity, matrix synthesis, and matrix gene expression profile were assessed and compared with fresh one.
The results showed ex vivo culturing of samples gradually destroyed the morphology.
Proteoglycan contents and gene expression were decreased and downregulated obviously.
However, immunohistochemical staining intensity and collagen type II gene expression were significantly enhanced and upregulated.
In contrast, these trends were reversed under constant compression.
These results indicated short-term static load stimulated the synthesis of type II collagen; however, constant compression led to progressive degeneration and specifically to proteoglycan.
Through this study a loading and organ-culturing system for ex vivo rabbit IVD motion segments was developed, which can be used to study the effects of mechanical stimulation on the biology of IVDs and the pathomechanics of IVD degeneration.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhan, Jia-Wen& Feng, Minshan& Zhu, Liguo& Zhang, Ping& Yu, Jie. 2016. Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097080
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhan, Jia-Wen…[et al.]. Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1097080
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhan, Jia-Wen& Feng, Minshan& Zhu, Liguo& Zhang, Ping& Yu, Jie. Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097080
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1097080
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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