Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture
Joint Authors
Zhu, Liguo
Feng, Minshan
Zhan, Jia-Wen
Zhang, Ping
Yu, Jie
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-10, 10 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-31
Country of Publication
Egypt
No. of Pages
10
Main Subjects
Abstract EN
The development of mechanically active culture systems helps in understanding of the role of mechanical stress in intervertebral disc (IVD) degeneration.
Motion segment cultures facilitate the application and control of mechanical loads.
The purpose of this study was to establish a culturing method for rabbit IVD motion segments to observe the effect of static load on the whole disc organ.
Segments were cultured in custom-made apparatuses under a constant, compressive load (3 kg) for 2 weeks.
Tissue integrity, matrix synthesis, and matrix gene expression profile were assessed and compared with fresh one.
The results showed ex vivo culturing of samples gradually destroyed the morphology.
Proteoglycan contents and gene expression were decreased and downregulated obviously.
However, immunohistochemical staining intensity and collagen type II gene expression were significantly enhanced and upregulated.
In contrast, these trends were reversed under constant compression.
These results indicated short-term static load stimulated the synthesis of type II collagen; however, constant compression led to progressive degeneration and specifically to proteoglycan.
Through this study a loading and organ-culturing system for ex vivo rabbit IVD motion segments was developed, which can be used to study the effects of mechanical stimulation on the biology of IVDs and the pathomechanics of IVD degeneration.
American Psychological Association (APA)
Zhan, Jia-Wen& Feng, Minshan& Zhu, Liguo& Zhang, Ping& Yu, Jie. 2016. Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture. BioMed Research International،Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097080
Modern Language Association (MLA)
Zhan, Jia-Wen…[et al.]. Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture. BioMed Research International No. 2016 (2016), pp.1-10.
https://search.emarefa.net/detail/BIM-1097080
American Medical Association (AMA)
Zhan, Jia-Wen& Feng, Minshan& Zhu, Liguo& Zhang, Ping& Yu, Jie. Effect of Static Load on the Nucleus Pulposus of Rabbit Intervertebral Disc Motion Segment in an Organ Culture. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-10.
https://search.emarefa.net/detail/BIM-1097080
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097080