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Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
المؤلفون المشاركون
Li, Yang
Wang, Yixin
Fang, Lichun
Fu, Jiayuan
Cui, Shuai
Zhao, Yingjie
Cui, Zhizhong
Chang, Shuang
Zhao, Peng
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-5، 5ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-19
دولة النشر
مصر
عدد الصفحات
5
التخصصات الرئيسية
الملخص EN
The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV.
In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification.
A CIAV strain (SD1403) was isolated and successfully identified.
Three overlapping genomic fragments were obtained by PCR amplification and sequencing.
The full genome sequence of the SD1403 strain was obtained by aligning the sequences.
The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains.
The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved.
Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics.
In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Li, Yang& Wang, Yixin& Fang, Lichun& Fu, Jiayuan& Cui, Shuai& Zhao, Yingjie…[et al.]. 2016. Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China. BioMed Research International،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1097715
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Li, Yang…[et al.]. Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China. BioMed Research International No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1097715
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Li, Yang& Wang, Yixin& Fang, Lichun& Fu, Jiayuan& Cui, Shuai& Zhao, Yingjie…[et al.]. Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1097715
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1097715
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
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تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر
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