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Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China
Joint Authors
Li, Yang
Wang, Yixin
Fang, Lichun
Fu, Jiayuan
Cui, Shuai
Zhao, Yingjie
Cui, Zhizhong
Chang, Shuang
Zhao, Peng
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-5, 5 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-19
Country of Publication
Egypt
No. of Pages
5
Main Subjects
Abstract EN
The antibody to chicken infectious anemia virus (CIAV) was positive in a specific pathogen-free (SPF) chicken population by ELISA test in our previous inspection, indicating a possible infection with CIAV.
In this study, blood samples collected from the SPF chickens were used to isolate CIAV by inoculating into MSB1 cells and PCR amplification.
A CIAV strain (SD1403) was isolated and successfully identified.
Three overlapping genomic fragments were obtained by PCR amplification and sequencing.
The full genome sequence of the SD1403 strain was obtained by aligning the sequences.
The genome of the SD1403 strain was 2293 bp with a nucleotide identity of 94.8% to 98.5% when compared with 30 referred CIAV strains.
The viral proteins VP2 and VP3 were highly conserved, but VP1 was not relatively conserved.
Both amino acids 139 and 144 of VP1 were glutamine, which was in accord with the low pathogenic characteristics.
In this study, we first reported that CIAV exists in Chinese SPF chicken populations and may be an important reason why attenuated vaccine can be contaminated with CIAV.
American Psychological Association (APA)
Li, Yang& Wang, Yixin& Fang, Lichun& Fu, Jiayuan& Cui, Shuai& Zhao, Yingjie…[et al.]. 2016. Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China. BioMed Research International،Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1097715
Modern Language Association (MLA)
Li, Yang…[et al.]. Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China. BioMed Research International No. 2016 (2016), pp.1-5.
https://search.emarefa.net/detail/BIM-1097715
American Medical Association (AMA)
Li, Yang& Wang, Yixin& Fang, Lichun& Fu, Jiayuan& Cui, Shuai& Zhao, Yingjie…[et al.]. Genomic Analysis of the Chicken Infectious Anemia Virus in a Specific Pathogen-Free Chicken Population in China. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-5.
https://search.emarefa.net/detail/BIM-1097715
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1097715