Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line

المؤلفون المشاركون

Zhang, Yufei
Shi, Jing
Liu, Shuying

المصدر

BioMed Research International

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-02-21

دولة النشر

مصر

عدد الصفحات

9

التخصصات الرئيسية

الطب البشري

الملخص EN

The primary sheep trophoblast cells (STCs) have a finite lifespan in culture.

This feature limits the scope for long-term in vitro studies with STCs.

This study was an attempt to establish and characterize a telomerase-immortalized sheep trophoblast cell line.

STCs were isolated and purified by using Percoll and specific immunoaffinity purification, respectively.

The purified STCs were transfected with a plasmid carrying sequences of human telomerase reverse transcriptase (hTERT) to create immortalized sheep trophoblast cell line (hTERT-STCs).

hTERT-STCs showed a stable expression of hTERT gene, serially passaged for a year, and showed active proliferation without signs of senescence.

Cytokeratin 7 (CK-7), secreted human chorionic gonadotrophin subunit β (CG-β), placental lactogen (PL), and endogenous jaagsiekte sheep retrovirus (enJSRV) envelope genes were expressed in hTERT-STCs.

Transwell cell invasion assay indicated that hTERT-STCs still possessed the same invasive characteristics as normal primary sheep trophoblast cells.

hTERT-STCs could not grow in soft agar and did not develop into tumors in nude mice.

In this study, we established a strain of immortalized sheep trophoblast cell line which could be gainfully employed in the future as an experimental model to study trophoblast cells with secretory function, invasive features, and probable biological function of enJSRV envelope genes.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Zhang, Yufei& Shi, Jing& Liu, Shuying. 2016. Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098222

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Zhang, Yufei…[et al.]. Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098222

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Zhang, Yufei& Shi, Jing& Liu, Shuying. Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098222

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1098222