Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line
Joint Authors
Zhang, Yufei
Shi, Jing
Liu, Shuying
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-02-21
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The primary sheep trophoblast cells (STCs) have a finite lifespan in culture.
This feature limits the scope for long-term in vitro studies with STCs.
This study was an attempt to establish and characterize a telomerase-immortalized sheep trophoblast cell line.
STCs were isolated and purified by using Percoll and specific immunoaffinity purification, respectively.
The purified STCs were transfected with a plasmid carrying sequences of human telomerase reverse transcriptase (hTERT) to create immortalized sheep trophoblast cell line (hTERT-STCs).
hTERT-STCs showed a stable expression of hTERT gene, serially passaged for a year, and showed active proliferation without signs of senescence.
Cytokeratin 7 (CK-7), secreted human chorionic gonadotrophin subunit β (CG-β), placental lactogen (PL), and endogenous jaagsiekte sheep retrovirus (enJSRV) envelope genes were expressed in hTERT-STCs.
Transwell cell invasion assay indicated that hTERT-STCs still possessed the same invasive characteristics as normal primary sheep trophoblast cells.
hTERT-STCs could not grow in soft agar and did not develop into tumors in nude mice.
In this study, we established a strain of immortalized sheep trophoblast cell line which could be gainfully employed in the future as an experimental model to study trophoblast cells with secretory function, invasive features, and probable biological function of enJSRV envelope genes.
American Psychological Association (APA)
Zhang, Yufei& Shi, Jing& Liu, Shuying. 2016. Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line. BioMed Research International،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098222
Modern Language Association (MLA)
Zhang, Yufei…[et al.]. Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line. BioMed Research International No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1098222
American Medical Association (AMA)
Zhang, Yufei& Shi, Jing& Liu, Shuying. Establishment and Characterization of a Telomerase-Immortalized Sheep Trophoblast Cell Line. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1098222
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098222