Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes

المؤلفون المشاركون

Tang, Li Hua
Tan, Qi
Bao, Da Peng
Zhang, Xue Hong
Jian, Hua Hua
Li, Yan
Yang, Rui heng
Wang, Ying

المصدر

BioMed Research International

العدد

المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.

الناشر

Hindawi Publishing Corporation

تاريخ النشر

2016-10-27

دولة النشر

مصر

عدد الصفحات

8

التخصصات الرئيسية

الطب البشري

الملخص EN

Light-induced brown film (BF) formation by the vegetative mycelium of Lentinula edodes is important for ensuring the quantity and quality of this edible mushroom.

Nevertheless, the molecular mechanism underlying this phenotype is still unclear.

In this study, a comparative proteomic analysis of mycelial BF formation in L.

edodes was performed.

Seventy-three protein spots with at least a twofold difference in abundance on two-dimensional electrophoresis (2DE) maps were observed, and 52 of them were successfully identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF/MS).

These proteins were classified into the following functional categories: small molecule metabolic processes (39%), response to oxidative stress (5%), and organic substance catabolic processes (5%), followed by oxidation-reduction processes (3%), single-organism catabolic processes (3%), positive regulation of protein complex assembly (3%), and protein metabolic processes (3%).

Interestingly, four of the proteins that were upregulated in response to light exposure were nucleoside diphosphate kinases.

To our knowledge, this is the first proteomic analysis of the mechanism of BF formation in L.

edodes.

Our data will provide a foundation for future detailed investigations of the proteins linked to BF formation.

نمط استشهاد جمعية علماء النفس الأمريكية (APA)

Tang, Li Hua& Tan, Qi& Bao, Da Peng& Zhang, Xue Hong& Jian, Hua Hua& Li, Yan…[et al.]. 2016. Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098230

نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)

Tang, Li Hua…[et al.]. Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1098230

نمط استشهاد الجمعية الطبية الأمريكية (AMA)

Tang, Li Hua& Tan, Qi& Bao, Da Peng& Zhang, Xue Hong& Jian, Hua Hua& Li, Yan…[et al.]. Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098230

نوع البيانات

مقالات

لغة النص

الإنجليزية

الملاحظات

Includes bibliographical references

رقم السجل

BIM-1098230