Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes
Joint Authors
Tang, Li Hua
Tan, Qi
Bao, Da Peng
Zhang, Xue Hong
Jian, Hua Hua
Li, Yan
Yang, Rui heng
Wang, Ying
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-10-27
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Light-induced brown film (BF) formation by the vegetative mycelium of Lentinula edodes is important for ensuring the quantity and quality of this edible mushroom.
Nevertheless, the molecular mechanism underlying this phenotype is still unclear.
In this study, a comparative proteomic analysis of mycelial BF formation in L.
edodes was performed.
Seventy-three protein spots with at least a twofold difference in abundance on two-dimensional electrophoresis (2DE) maps were observed, and 52 of them were successfully identified by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry (MALDI-TOF/TOF/MS).
These proteins were classified into the following functional categories: small molecule metabolic processes (39%), response to oxidative stress (5%), and organic substance catabolic processes (5%), followed by oxidation-reduction processes (3%), single-organism catabolic processes (3%), positive regulation of protein complex assembly (3%), and protein metabolic processes (3%).
Interestingly, four of the proteins that were upregulated in response to light exposure were nucleoside diphosphate kinases.
To our knowledge, this is the first proteomic analysis of the mechanism of BF formation in L.
edodes.
Our data will provide a foundation for future detailed investigations of the proteins linked to BF formation.
American Psychological Association (APA)
Tang, Li Hua& Tan, Qi& Bao, Da Peng& Zhang, Xue Hong& Jian, Hua Hua& Li, Yan…[et al.]. 2016. Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098230
Modern Language Association (MLA)
Tang, Li Hua…[et al.]. Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1098230
American Medical Association (AMA)
Tang, Li Hua& Tan, Qi& Bao, Da Peng& Zhang, Xue Hong& Jian, Hua Hua& Li, Yan…[et al.]. Comparative Proteomic Analysis of Light-Induced Mycelial Brown Film Formation in Lentinula edodes. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1098230
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098230