Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway
المؤلفون المشاركون
Lu, Jingjing
Xu, Ying
Wei, Xuan
Zhao, Zhe
Xue, Jing
Liu, Peishu
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-16، 16ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-12-20
دولة النشر
مصر
عدد الصفحات
16
التخصصات الرئيسية
الملخص EN
Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy.
Despite the anticancer capabilities of emodin observed in many cancers, including EOC, the underlying molecular mechanism remains to be elucidated.
A crucial link has been discovered between the acquisition of metastatic traits and the epithelial-mesenchymal transition (EMT).
The present study aimed to determine whether emodin could inhibit the EMT of EOC cells and explore the underlying mechanism.
The CCK-8 assay and transwell assay showed that emodin effectively repressed the abilities of proliferation, invasion, and migration in A2780 and SK-OV-3 cells.
The Western blot showed that emodin upregulated epithelial markers (E-cadherin and Claudin) while it downregulated mesenchymal markers (N-cadherin and Vimentin) and transcription factor (Slug) in a dose-dependent fashion.
After transfection of siRNA-Slug, both Slug and N-cadherin were downregulated in EOC cells while E-cadherin was upregulated, which was intensified by emodin.
Besides, emodin decreased the expression of ILK, p-GSK-3β, β-catenin, and Slug.
Transfection of siRNA-ILK also achieved the same effects, which was further strengthened by following emodin treatment.
Nevertheless, SB216763, an inhibitor of GSK-3β, could reverse the effects of emodin except for ILK expression.
These findings suggest that emodin inhibited the EMT of EOC cells via ILK/GSK-3β/Slug signaling pathway.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Lu, Jingjing& Xu, Ying& Wei, Xuan& Zhao, Zhe& Xue, Jing& Liu, Peishu. 2016. Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway. BioMed Research International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1098421
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Lu, Jingjing…[et al.]. Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway. BioMed Research International No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1098421
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Lu, Jingjing& Xu, Ying& Wei, Xuan& Zhao, Zhe& Xue, Jing& Liu, Peishu. Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1098421
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1098421
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر