Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway
Joint Authors
Lu, Jingjing
Xu, Ying
Wei, Xuan
Zhao, Zhe
Xue, Jing
Liu, Peishu
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-16, 16 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-12-20
Country of Publication
Egypt
No. of Pages
16
Main Subjects
Abstract EN
Epithelial ovarian cancer (EOC) is the most lethal gynecologic malignancy.
Despite the anticancer capabilities of emodin observed in many cancers, including EOC, the underlying molecular mechanism remains to be elucidated.
A crucial link has been discovered between the acquisition of metastatic traits and the epithelial-mesenchymal transition (EMT).
The present study aimed to determine whether emodin could inhibit the EMT of EOC cells and explore the underlying mechanism.
The CCK-8 assay and transwell assay showed that emodin effectively repressed the abilities of proliferation, invasion, and migration in A2780 and SK-OV-3 cells.
The Western blot showed that emodin upregulated epithelial markers (E-cadherin and Claudin) while it downregulated mesenchymal markers (N-cadherin and Vimentin) and transcription factor (Slug) in a dose-dependent fashion.
After transfection of siRNA-Slug, both Slug and N-cadherin were downregulated in EOC cells while E-cadherin was upregulated, which was intensified by emodin.
Besides, emodin decreased the expression of ILK, p-GSK-3β, β-catenin, and Slug.
Transfection of siRNA-ILK also achieved the same effects, which was further strengthened by following emodin treatment.
Nevertheless, SB216763, an inhibitor of GSK-3β, could reverse the effects of emodin except for ILK expression.
These findings suggest that emodin inhibited the EMT of EOC cells via ILK/GSK-3β/Slug signaling pathway.
American Psychological Association (APA)
Lu, Jingjing& Xu, Ying& Wei, Xuan& Zhao, Zhe& Xue, Jing& Liu, Peishu. 2016. Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway. BioMed Research International،Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1098421
Modern Language Association (MLA)
Lu, Jingjing…[et al.]. Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway. BioMed Research International No. 2016 (2016), pp.1-16.
https://search.emarefa.net/detail/BIM-1098421
American Medical Association (AMA)
Lu, Jingjing& Xu, Ying& Wei, Xuan& Zhao, Zhe& Xue, Jing& Liu, Peishu. Emodin Inhibits the Epithelial to Mesenchymal Transition of Epithelial Ovarian Cancer Cells via ILKGSK-3βSlug Signaling Pathway. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-16.
https://search.emarefa.net/detail/BIM-1098421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1098421