Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System
المؤلفون المشاركون
Zhang, Xu
Zhang, Shuangxi
Wang, Jun
Zi, Jing
Wang, Jianhui
Chen, Shaolin
Wan, Yi
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-11-24
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
The plant-specific sucrose nonfermenting 1-related protein kinase 2 (SnRK2) family is considered an important regulator of plant responses to abiotic stresses such as drought, cold, salinity, and nutrition deficiency.
However, little information is available on how SnRK2s regulate sulfur deprivation responses in Arabidopsis.
Large-scale production of SnRK2 kinases in vitro can help to elucidate the biochemical properties and physiological functions of this protein family.
However, heterogenous expression of SnRK2s usually leads to inactive proteins.
In this study, we expressed a recombinant Arabidopsis SnRK2.1 in a modified E.
coli cell-free system, which combined two kinds of extracts allowing for a convenient and affordable protein preparation.
The recombinant SnRK2.1 was produced in large-scale and the autophosphorylation activity of purified SnRK2.1 was characterized, allowing for further biochemical and substrate binding analysis in sulfur signaling.
The application of this improved E.
coli cell-free system provides us a promising and convenient platform to enhance expression of the target proteins economically.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Zhang, Xu& Zhang, Shuangxi& Wang, Jun& Zi, Jing& Wang, Jianhui& Chen, Shaolin…[et al.]. 2016. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099305
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Zhang, Xu…[et al.]. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099305
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Zhang, Xu& Zhang, Shuangxi& Wang, Jun& Zi, Jing& Wang, Jianhui& Chen, Shaolin…[et al.]. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099305
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1099305
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر