Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System
Joint Authors
Zhang, Xu
Zhang, Shuangxi
Wang, Jun
Zi, Jing
Wang, Jianhui
Chen, Shaolin
Wan, Yi
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-11-24
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The plant-specific sucrose nonfermenting 1-related protein kinase 2 (SnRK2) family is considered an important regulator of plant responses to abiotic stresses such as drought, cold, salinity, and nutrition deficiency.
However, little information is available on how SnRK2s regulate sulfur deprivation responses in Arabidopsis.
Large-scale production of SnRK2 kinases in vitro can help to elucidate the biochemical properties and physiological functions of this protein family.
However, heterogenous expression of SnRK2s usually leads to inactive proteins.
In this study, we expressed a recombinant Arabidopsis SnRK2.1 in a modified E.
coli cell-free system, which combined two kinds of extracts allowing for a convenient and affordable protein preparation.
The recombinant SnRK2.1 was produced in large-scale and the autophosphorylation activity of purified SnRK2.1 was characterized, allowing for further biochemical and substrate binding analysis in sulfur signaling.
The application of this improved E.
coli cell-free system provides us a promising and convenient platform to enhance expression of the target proteins economically.
American Psychological Association (APA)
Zhang, Xu& Zhang, Shuangxi& Wang, Jun& Zi, Jing& Wang, Jianhui& Chen, Shaolin…[et al.]. 2016. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099305
Modern Language Association (MLA)
Zhang, Xu…[et al.]. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099305
American Medical Association (AMA)
Zhang, Xu& Zhang, Shuangxi& Wang, Jun& Zi, Jing& Wang, Jianhui& Chen, Shaolin…[et al.]. Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099305
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099305