Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production
المؤلفون المشاركون
Xue, Feiyan
Guo, Huili
Hu, Yingying
Liu, Ran
Huang, Lina
Lv, Heshu
Liu, Chunmei
Yang, Mingfeng
Ma, Lanqing
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-8، 8ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-08-15
دولة النشر
مصر
عدد الصفحات
8
التخصصات الرئيسية
الملخص EN
Salidroside, a plant secondary metabolite in Rhodiola, has been demonstrated to have several adaptogenic properties as a medicinal herb.
Due to the limitation of plant source, microbial production of salidroside by expression of plant uridine diphosphate glycosyltransferase (UGT) is promising.
However, glycoside production usually remains hampered by poor expression of plant UGTs in microorganisms.
Herein, we achieved salidroside production by expression of Rhodiola UGT72B14 in Escherichia coli (E.
coli) and codon optimization was accordingly applied.
UGT72B14 expression was optimized by changing 278 nucleotides and decreasing the G+C content to 51.05% without altering the amino acid sequence.
The effect of codon optimization on UGT72B14 catalysis for salidroside production was assessed both in vitro and in vivo.
In vitro, salidroside production by codon-optimized UGT72B14 is enhanced because of a significantly improved protein yield (increased by 4.8-fold) and an equivalently high activity as demonstrated by similar kinetic parameters ( K M and V m a x ), compared to that by wild-type protein.
In vivo, both batch and fed-batch cultivation using the codon-optimized gene resulted in a significant increase in salidroside production, which was up to 6.7 mg/L increasing 3.2-fold over the wild-type UGT72B14.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Xue, Feiyan& Guo, Huili& Hu, Yingying& Liu, Ran& Huang, Lina& Lv, Heshu…[et al.]. 2016. Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099415
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Xue, Feiyan…[et al.]. Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099415
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Xue, Feiyan& Guo, Huili& Hu, Yingying& Liu, Ran& Huang, Lina& Lv, Heshu…[et al.]. Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099415
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1099415
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر