Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production
Joint Authors
Xue, Feiyan
Guo, Huili
Hu, Yingying
Liu, Ran
Huang, Lina
Lv, Heshu
Liu, Chunmei
Yang, Mingfeng
Ma, Lanqing
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-8, 8 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-08-15
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
Salidroside, a plant secondary metabolite in Rhodiola, has been demonstrated to have several adaptogenic properties as a medicinal herb.
Due to the limitation of plant source, microbial production of salidroside by expression of plant uridine diphosphate glycosyltransferase (UGT) is promising.
However, glycoside production usually remains hampered by poor expression of plant UGTs in microorganisms.
Herein, we achieved salidroside production by expression of Rhodiola UGT72B14 in Escherichia coli (E.
coli) and codon optimization was accordingly applied.
UGT72B14 expression was optimized by changing 278 nucleotides and decreasing the G+C content to 51.05% without altering the amino acid sequence.
The effect of codon optimization on UGT72B14 catalysis for salidroside production was assessed both in vitro and in vivo.
In vitro, salidroside production by codon-optimized UGT72B14 is enhanced because of a significantly improved protein yield (increased by 4.8-fold) and an equivalently high activity as demonstrated by similar kinetic parameters ( K M and V m a x ), compared to that by wild-type protein.
In vivo, both batch and fed-batch cultivation using the codon-optimized gene resulted in a significant increase in salidroside production, which was up to 6.7 mg/L increasing 3.2-fold over the wild-type UGT72B14.
American Psychological Association (APA)
Xue, Feiyan& Guo, Huili& Hu, Yingying& Liu, Ran& Huang, Lina& Lv, Heshu…[et al.]. 2016. Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production. BioMed Research International،Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099415
Modern Language Association (MLA)
Xue, Feiyan…[et al.]. Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production. BioMed Research International No. 2016 (2016), pp.1-8.
https://search.emarefa.net/detail/BIM-1099415
American Medical Association (AMA)
Xue, Feiyan& Guo, Huili& Hu, Yingying& Liu, Ran& Huang, Lina& Lv, Heshu…[et al.]. Expression of Codon-Optimized Plant Glycosyltransferase UGT72B14 in Escherichia coli Enhances Salidroside Production. BioMed Research International. 2016. Vol. 2016, no. 2016, pp.1-8.
https://search.emarefa.net/detail/BIM-1099415
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1099415