EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method
المؤلفون المشاركون
Nukiwa, Toshohiro
Watanabe, Kana
Fukuhara, Tatsuro
Tsukita, Yoko
Morita, Mami
Suzuki, Aya
Tanaka, Nobuyuki
Terasaki, Hiroshi
Maemondo, Makoto
المصدر
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-7، 7ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-07-13
دولة النشر
مصر
عدد الصفحات
7
التخصصات الرئيسية
الملخص EN
Introduction.
Rebiopsies have become more crucial in non-small cell lung cancer (NSCLC).
Instead of invasive biopsies, development of collecting biological data of the tumor from blood samples is expected.
We conducted a prospective study to assess the feasibility of detection of epidermal growth factor receptor (EGFR) mutation in plasma samples.
Method.
NSCLC patients harboring EGFR activating mutations, who were going to receive EGFR-tyrosine kinase inhibitors (TKIs) as first-line treatment, were enrolled in this study.
Plasma EGFR activating mutations and the T790M resistance mutation were analyzed by an improved PNA-LNA PCR clamp method, characterized by a 10-fold or more sensitivity compared with the original methods.
Result.
Six patients with wild-type EGFR and 24 patients with EGFR mutations were enrolled in this study.
Pretreatment plasma samples achieved sensitivity of 79%.
The 6 patients with wild-type EGFR were all negative for plasma EGFR mutations.
At the time of disease progression, plasma T790M mutation was detected in 8 of 16 cases.
Absence of T790M before and during TKI treatment and disappearance of activating mutations during TKI treatment were considered as predictors of EGFR-TKIs efficacy.
Conclusion.
We were able to detect EGFR mutations in plasma samples by using an improved PNA-LNA PCR clamp method.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Watanabe, Kana& Fukuhara, Tatsuro& Tsukita, Yoko& Morita, Mami& Suzuki, Aya& Tanaka, Nobuyuki…[et al.]. 2016. EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method. Canadian Respiratory Journal،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103207
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Watanabe, Kana…[et al.]. EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method. Canadian Respiratory Journal No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1103207
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Watanabe, Kana& Fukuhara, Tatsuro& Tsukita, Yoko& Morita, Mami& Suzuki, Aya& Tanaka, Nobuyuki…[et al.]. EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method. Canadian Respiratory Journal. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103207
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1103207
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر