EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method
Joint Authors
Nukiwa, Toshohiro
Watanabe, Kana
Fukuhara, Tatsuro
Tsukita, Yoko
Morita, Mami
Suzuki, Aya
Tanaka, Nobuyuki
Terasaki, Hiroshi
Maemondo, Makoto
Source
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-7, 7 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-07-13
Country of Publication
Egypt
No. of Pages
7
Main Subjects
Abstract EN
Introduction.
Rebiopsies have become more crucial in non-small cell lung cancer (NSCLC).
Instead of invasive biopsies, development of collecting biological data of the tumor from blood samples is expected.
We conducted a prospective study to assess the feasibility of detection of epidermal growth factor receptor (EGFR) mutation in plasma samples.
Method.
NSCLC patients harboring EGFR activating mutations, who were going to receive EGFR-tyrosine kinase inhibitors (TKIs) as first-line treatment, were enrolled in this study.
Plasma EGFR activating mutations and the T790M resistance mutation were analyzed by an improved PNA-LNA PCR clamp method, characterized by a 10-fold or more sensitivity compared with the original methods.
Result.
Six patients with wild-type EGFR and 24 patients with EGFR mutations were enrolled in this study.
Pretreatment plasma samples achieved sensitivity of 79%.
The 6 patients with wild-type EGFR were all negative for plasma EGFR mutations.
At the time of disease progression, plasma T790M mutation was detected in 8 of 16 cases.
Absence of T790M before and during TKI treatment and disappearance of activating mutations during TKI treatment were considered as predictors of EGFR-TKIs efficacy.
Conclusion.
We were able to detect EGFR mutations in plasma samples by using an improved PNA-LNA PCR clamp method.
American Psychological Association (APA)
Watanabe, Kana& Fukuhara, Tatsuro& Tsukita, Yoko& Morita, Mami& Suzuki, Aya& Tanaka, Nobuyuki…[et al.]. 2016. EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method. Canadian Respiratory Journal،Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103207
Modern Language Association (MLA)
Watanabe, Kana…[et al.]. EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method. Canadian Respiratory Journal No. 2016 (2016), pp.1-7.
https://search.emarefa.net/detail/BIM-1103207
American Medical Association (AMA)
Watanabe, Kana& Fukuhara, Tatsuro& Tsukita, Yoko& Morita, Mami& Suzuki, Aya& Tanaka, Nobuyuki…[et al.]. EGFR Mutation Analysis of Circulating Tumor DNA Using an Improved PNA-LNA PCR Clamp Method. Canadian Respiratory Journal. 2016. Vol. 2016, no. 2016, pp.1-7.
https://search.emarefa.net/detail/BIM-1103207
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103207