Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
المؤلفون المشاركون
Bolduc, Stéphane
Chabaud, Stéphane
المصدر
Dermatology Research and Practice
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-9، 9ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-03-09
دولة النشر
مصر
عدد الصفحات
9
التخصصات الرئيسية
الملخص EN
Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment.
Such tissue reorganization could have impact on cell behaviour and/or more global biological processes.
Tissue engineering provides accurate study model by mimicking the skin and it allows the construction of versatile tridimensional models using human cells.
It also avoids the use of animals, which gave sometimes nontranslatable results.
Among the various techniques existing, the self-assembly method allows production of a near native skin, free of exogenous material.
After cultivating human dermal fibroblasts in the presence of ascorbate during two weeks, a reseeding of these cells takes place after elevation of the resulting stroma on a permeable ring and culture pursued for another two weeks.
This protocol induces a clear realignment of matrix fibres and cells parallel to the horizon.
The thickness of this stretched reconstructed tissue is reduced compared to the stroma produced by the standard technique.
Cell count is also reduced.
In conclusion, a new, easy, and inexpensive method to produce aligned tissue free of exogenous material could be used for fundamental research applications in dermatology.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Chabaud, Stéphane& Bolduc, Stéphane. 2016. Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique. Dermatology Research and Practice،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103840
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Chabaud, Stéphane& Bolduc, Stéphane. Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique. Dermatology Research and Practice No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1103840
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Chabaud, Stéphane& Bolduc, Stéphane. Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique. Dermatology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103840
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1103840
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر