Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique
Joint Authors
Bolduc, Stéphane
Chabaud, Stéphane
Source
Dermatology Research and Practice
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-9, 9 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-03-09
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
Many pathologies of skin, especially ageing and cancer, involve modifications in the matrix alignment.
Such tissue reorganization could have impact on cell behaviour and/or more global biological processes.
Tissue engineering provides accurate study model by mimicking the skin and it allows the construction of versatile tridimensional models using human cells.
It also avoids the use of animals, which gave sometimes nontranslatable results.
Among the various techniques existing, the self-assembly method allows production of a near native skin, free of exogenous material.
After cultivating human dermal fibroblasts in the presence of ascorbate during two weeks, a reseeding of these cells takes place after elevation of the resulting stroma on a permeable ring and culture pursued for another two weeks.
This protocol induces a clear realignment of matrix fibres and cells parallel to the horizon.
The thickness of this stretched reconstructed tissue is reduced compared to the stroma produced by the standard technique.
Cell count is also reduced.
In conclusion, a new, easy, and inexpensive method to produce aligned tissue free of exogenous material could be used for fundamental research applications in dermatology.
American Psychological Association (APA)
Chabaud, Stéphane& Bolduc, Stéphane. 2016. Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique. Dermatology Research and Practice،Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103840
Modern Language Association (MLA)
Chabaud, Stéphane& Bolduc, Stéphane. Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique. Dermatology Research and Practice No. 2016 (2016), pp.1-9.
https://search.emarefa.net/detail/BIM-1103840
American Medical Association (AMA)
Chabaud, Stéphane& Bolduc, Stéphane. Production of a Self-Aligned Scaffold, Free of Exogenous Material, from Dermal Fibroblasts Using the Self-Assembly Technique. Dermatology Research and Practice. 2016. Vol. 2016, no. 2016, pp.1-9.
https://search.emarefa.net/detail/BIM-1103840
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1103840