Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A
المؤلفون المشاركون
Ortiz, Carmen
Morales, Luisa
Sastre, Miguel
Haskins, William E.
Matta, Jaime
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-13، 13ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-05-11
دولة النشر
مصر
عدد الصفحات
13
التخصصات الرئيسية
الملخص EN
Sandalwood essential oil (SEO) is extracted from Santalum trees.
Although α-santalol, a main constituent of SEO, has been studied as a chemopreventive agent, the genotoxic activity of the whole oil in human breast cell lines is still unknown.
The main objective of this study was to assess the cytotoxic and genotoxic effects of SEO in breast adenocarcinoma (MCF-7) and nontumorigenic breast epithelial (MCF-10A) cells.
Proteins associated with SEO genotoxicity were identified using a proteomics approach.
Commercially available, high-purity, GC/MS characterized SEO was used to perform the experiments.
The main constituents reported in the oil were (Z)-α-santalol (25.34%), (Z)-nuciferol (18.34%), (E)-β-santalol (10.97%), and (E)-nuciferol (10.46%).
Upon exposure to SEO (2–8 μg/mL) for 24 hours, cell proliferation was determined by the MTT assay.
Alkaline and neutral comet assays were used to assess genotoxicity.
SEO exposure induced single- and double-strand breaks selectively in the DNA of MCF-7 cells.
Quantitative LC/MS-based proteomics allowed identification of candidate proteins involved in this response: Ku70 ( p = 1.37 E - 2 ), Ku80 ( p = 5.8 E - 3 ), EPHX1 ( p = 3.3 E - 3 ), and 14-3-3ζ ( p = 4.0 E - 4 ).
These results provide the first evidence that SEO is genotoxic and capable of inducing DNA single- and double-strand breaks in MCF-7 cells.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Ortiz, Carmen& Morales, Luisa& Sastre, Miguel& Haskins, William E.& Matta, Jaime. 2016. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104095
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Ortiz, Carmen…[et al.]. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1104095
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Ortiz, Carmen& Morales, Luisa& Sastre, Miguel& Haskins, William E.& Matta, Jaime. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104095
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104095
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر