Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A
Joint Authors
Ortiz, Carmen
Morales, Luisa
Sastre, Miguel
Haskins, William E.
Matta, Jaime
Source
Evidence-Based Complementary and Alternative Medicine
Issue
Vol. 2016, Issue 2016 (31 Dec. 2016), pp.1-13, 13 p.
Publisher
Hindawi Publishing Corporation
Publication Date
2016-05-11
Country of Publication
Egypt
No. of Pages
13
Main Subjects
Abstract EN
Sandalwood essential oil (SEO) is extracted from Santalum trees.
Although α-santalol, a main constituent of SEO, has been studied as a chemopreventive agent, the genotoxic activity of the whole oil in human breast cell lines is still unknown.
The main objective of this study was to assess the cytotoxic and genotoxic effects of SEO in breast adenocarcinoma (MCF-7) and nontumorigenic breast epithelial (MCF-10A) cells.
Proteins associated with SEO genotoxicity were identified using a proteomics approach.
Commercially available, high-purity, GC/MS characterized SEO was used to perform the experiments.
The main constituents reported in the oil were (Z)-α-santalol (25.34%), (Z)-nuciferol (18.34%), (E)-β-santalol (10.97%), and (E)-nuciferol (10.46%).
Upon exposure to SEO (2–8 μg/mL) for 24 hours, cell proliferation was determined by the MTT assay.
Alkaline and neutral comet assays were used to assess genotoxicity.
SEO exposure induced single- and double-strand breaks selectively in the DNA of MCF-7 cells.
Quantitative LC/MS-based proteomics allowed identification of candidate proteins involved in this response: Ku70 ( p = 1.37 E - 2 ), Ku80 ( p = 5.8 E - 3 ), EPHX1 ( p = 3.3 E - 3 ), and 14-3-3ζ ( p = 4.0 E - 4 ).
These results provide the first evidence that SEO is genotoxic and capable of inducing DNA single- and double-strand breaks in MCF-7 cells.
American Psychological Association (APA)
Ortiz, Carmen& Morales, Luisa& Sastre, Miguel& Haskins, William E.& Matta, Jaime. 2016. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104095
Modern Language Association (MLA)
Ortiz, Carmen…[et al.]. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-13.
https://search.emarefa.net/detail/BIM-1104095
American Medical Association (AMA)
Ortiz, Carmen& Morales, Luisa& Sastre, Miguel& Haskins, William E.& Matta, Jaime. Cytotoxicity and Genotoxicity Assessment of Sandalwood Essential Oil in Human Breast Cell Lines MCF-7 and MCF-10A. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-13.
https://search.emarefa.net/detail/BIM-1104095
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references
Record ID
BIM-1104095