Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model
المؤلفون المشاركون
Mao, Bing
Liu, Wei
Jiang, Hong-li
Cai, Lin-li
Yan, Min
Dong, Shou-jin
المصدر
Evidence-Based Complementary and Alternative Medicine
العدد
المجلد 2016، العدد 2016 (31 ديسمبر/كانون الأول 2016)، ص ص. 1-15، 15ص.
الناشر
Hindawi Publishing Corporation
تاريخ النشر
2016-06-05
دولة النشر
مصر
عدد الصفحات
15
التخصصات الرئيسية
الملخص EN
Background.
Tanreqing injection (TRQ) is a commonly used herbal patent medicine for treating inflammatory airway diseases in view of its outstanding anti-inflammatory properties.
In this study, we explored the signaling pathways involved in contributions of TRQ to LPS-induced airway inflammation in rats.
Methods/Design.
Adult male Sprague Dawley (SD) rats randomly divided into different groups received intratracheal instillation of LPS and/or intraperitoneal injection of TRQ.
Bronchoalveolar Lavage Fluid (BALF) and lung samples were collected at 24 h, 48 h, and 96 h after TRQ administration.
Protein and mRNA levels of tumor necrosis factor- (TNF-) α, Interleukin- (IL-) 1β, IL-6, and IL-8 in BALF and lung homogenate were observed by ELISA and real-time PCR, respectively.
Lung sections were stained for p38 MAPK and NF-κB detection by immunohistochemistry.
Phospho-p38 MAPK, phosphor-extracellular signal-regulated kinases ERK1/2, phospho-SAPK/JNK, phospho-NF-κB p65, phospho-IKKα/β, and phospho-IκB-α were measured by western blot analysis.
Results.
The results showed that TRQ significantly counteracted LPS-stimulated release of TNF-α, IL-1β, IL-6, and IL-8, attenuated cells influx in BALF, mitigated mucus hypersecretion, suppressed phosphorylation of NF-κB p65, IκB-α, ΙKKα/β, ERK1/2, JNK, and p38 MAPK, and inhibited p38 MAPK and NF-κB p65 expression in rat lungs.
Conclusions.
Results of the current research indicate that TRQ possesses potent exhibitory effects in LPS-induced airway inflammation by, at least partially, suppressing the MAPKs and NF-κB signaling pathways, in a general dose-dependent manner.
نمط استشهاد جمعية علماء النفس الأمريكية (APA)
Liu, Wei& Jiang, Hong-li& Cai, Lin-li& Yan, Min& Dong, Shou-jin& Mao, Bing. 2016. Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model. Evidence-Based Complementary and Alternative Medicine،Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1104201
نمط استشهاد الجمعية الأمريكية للغات الحديثة (MLA)
Liu, Wei…[et al.]. Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model. Evidence-Based Complementary and Alternative Medicine No. 2016 (2016), pp.1-15.
https://search.emarefa.net/detail/BIM-1104201
نمط استشهاد الجمعية الطبية الأمريكية (AMA)
Liu, Wei& Jiang, Hong-li& Cai, Lin-li& Yan, Min& Dong, Shou-jin& Mao, Bing. Tanreqing Injection Attenuates Lipopolysaccharide-Induced Airway Inflammation through MAPKNF-κB Signaling Pathways in Rats Model. Evidence-Based Complementary and Alternative Medicine. 2016. Vol. 2016, no. 2016, pp.1-15.
https://search.emarefa.net/detail/BIM-1104201
نوع البيانات
مقالات
لغة النص
الإنجليزية
الملاحظات
Includes bibliographical references
رقم السجل
BIM-1104201
قاعدة معامل التأثير والاستشهادات المرجعية العربي "ارسيف Arcif"
أضخم قاعدة بيانات عربية للاستشهادات المرجعية للمجلات العلمية المحكمة الصادرة في العالم العربي
تقوم هذه الخدمة بالتحقق من التشابه أو الانتحال في الأبحاث والمقالات العلمية والأطروحات الجامعية والكتب والأبحاث باللغة العربية، وتحديد درجة التشابه أو أصالة الأعمال البحثية وحماية ملكيتها الفكرية. تعرف اكثر